Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
Carbonic Anhydrase IX Monoclonal Antibody (GT546)
FIGURE: 1 / 4
Carbonic Anhydrase IX Antibody (MA5-31495) in WB
Carbonic Anhydrase IX Monoclonal Antibody (GT546) detects Carbonic Anhydrase IX protein by Western blot analysis. A. 30 µg HeLa whole cell lysate/extract (untreated). B. 30 µg HeLa whole cell lysate/extract (1% O2 treatment for 24 hr). C. 30 µg HeLa whole cell lysate/extract (1% O2 treatment for 48 hr).7.5 % SDS-PAGE. Carbonic Anhydrase IX Monoclonal Antibody (GT546) (Product # MA5-31495) dilution: 1:1,000.
Product Details
MA5-31495
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
GT546
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human Carbonic Anhydrase IX.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2787126
Product Specific Information
Keep as concentrated solution.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Carbonic anhydrase (CA) is an enzyme that assists rapid interconversion of carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. It is abundant in all mammalian tissues. There are many genes that are inducible by hypoxia, via HIF-1 alpha. CA IX is one of the most inducible genes because of its stability and location within the membrane. Carbonic anhydrases have a widespread role in regulating pH in normal tissues, by regulating hydrogen ion (H+) flux. The pH is important in cell death under hypoxia, thus a blockade of CA IX results in increased cell death under hypoxia. Therefore, CA IX has become a reliable histochemical marker of hypoxia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: CA-9; CA-IX; Carbonate dehydratase IX; Carbonic anhydrase 9; Carbonic anhydrase IX; carbonic dehydratase; Membrane antigen MN; P54 / 58N; P54/58N; pMW1; RCC-associated antigen G250; RCC-associated protein G250; Renal cell carcinoma-associated antigen G250
Gene Aliases: CA9; CAIX; G250; MN
UniProt ID: (Human) Q16790
Entrez Gene ID: (Human) 768
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support