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Invitrogen
Catenin alpha-1 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 4
Catenin alpha-1 Antibody (PA1-25081) in WB
Western blot was performed using Anti-Catenin alpha-1 Polyclonal Antibody(Product # PA1-25081) and a 100kDa band corresponding to Catenin alpha-1 was observed across cell lines and tissue extracts tested except PC-3 and Daudi which is reported to be negative. A non-specific band around 160kDA has also been observed in cell lines. Whole Cell Extract-WCL (30 µg lysate) of A-431 (Lane 1), HeLa (Lane 2), MCF7 (Lane 3), LNCaP (Lane 4), PC-3 (Lane 5), Daudi (Lane 6), NIH/3T3 (Lane 7), PC-12 (Lane 8) and tissue extracts of Mouse Lung (Lane 9), Mo... View More
Product Details
PA1-25081
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse,
Rat,
Xenopus
Published species
Xenopus
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide, conjugated to KLH, corresponding to residues E(873) K Q D E T Q T K I K R A S Q(887) of human alpha 1 Catenin.
Conjugate
Form
Liquid
Purification
IgG fraction
Storage buffer
PBS, pH 7.4
Contains
15mM sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_779553
Product Specific Information
Recommended positive controls: A431 and cytosolic fraction of rat embryonic brain, MDCK, MCF-7.
PA1-25081 is expected to cross react with mouse (100% conserved) and Xenopus laevis (100% conserved) due to sequence homology.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherence junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 102 kDa cadherin-associated protein; alpha E catenin; Alpha E-catenin; alpha(E)-catenin; alpha-E-catenin; cadherin associated protein; Cadherin-associated protein; CAP102; catenin (cadherin associated protein), alpha 1; catenin (cadherin-associated protein), alpha 1, 102kDa; catenin alpha 1; Catenin alpha-1; catenin, alpha 1; Renal carcinoma antigen NY-REN-13
Gene Aliases: 2010010M04Rik; AA517462; AI988031; CAP102; Catna1; CTNNA1; MDPT2; XELAEV_18020018mg
UniProt ID: (Human) P35221, (Mouse) P26231
Entrez Gene ID: (Dog) 474698, (Human) 1495, (Rat) 307505, (Mouse) 12385, (Xenopus) 399303
ovarian follicle development
actin filament organization
cell adhesion
establishment or maintenance of cell polarity
negative regulation of neuroblast proliferation
aging
male gonad development
gap junction assembly
axon regeneration
adherens junction organization
cellular protein localization
odontogenesis of dentin-containing tooth
apical junction assembly
response to estrogen
positive regulation of smoothened signaling pathway
positive regulation of muscle cell differentiation
protein heterooligomerization
cellular response to indole-3-methanol
epithelial cell-cell adhesion
negative regulation of cell motility
negative regulation of integrin-mediated signaling pathway
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
positive regulation of extrinsic apoptotic signaling pathway in absence of ligand
response to organic cyclic compound
regulation of cell proliferation
negative regulation of apoptotic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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