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Invitrogen
Collagen IV Monoclonal Antibody (1042), eFluor™ 660, eBioscience™
Product Details
50-9871-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG2b, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
1042
Conjugate
Excitation/Emission Max
651/668 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2574404
Product Specific Information
Description: This monoclonal antibody 1042 recognizes human collagen Type IV. Collagens are the main components of the extracellular matrix and provide the tensile strength to tissues. There are four main types of collagen (I, II, III and IV) which contribute to the connective tissue. Expression of type IV is restricted to the basal lamina. It contains two alpha-1 and one alpha-2-like chains. Collagen Type IV is associated with disorders such as Alport syndrome and Goodpasture's syndrome.
Applications Reported: This 1042 antibody has been reported for use in immunohistochemical staining, immunohistochemical staining of frozen tissue sections, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.
Applications Tested: This 1042 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Collagen IV is a type of collagen protein that is a major component of the basement membrane in various tissues, including the kidneys, lungs, and skin. It forms a network-like structure that provides support and stability to the surrounding tissue. Collagen IV is composed of three alpha chains, which are encoded by the COL4A1, COL4A2, and COL4A3 genes. Mutations in these genes can lead to various genetic disorders, such as Alport syndrome and Goodpasture syndrome, which affect the kidneys and lungs, respectively.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: arresten; COL4A1 NC1 domain; Collagen 4; Collagen alpha-1(IV) chain; Collagen alpha-2(IV) chain; Collagen alpha-3(IV) chain; Collagen alpha-4(IV) chain; Collagen alpha-5(IV) chain; Collagen alpha-6(IV) chain; collagen IV, alpha-1 polypeptide; collagen IV, alpha-3 polypeptide; Collagen IV, alpha-4 polypeptide; collagen IV, alpha-5 polypeptide; collagen IV, alpha-6 polypeptide; collagen of basement membrane, alpha-1 chain; collagen of basement membrane, alpha-4 chain; collagen of basement membrane, alpha-5 chain; collagen of basement membrane, alpha-6; collagen, type IV, alpha 1; collagen, type IV, alpha 3 (Goodpasture antigen); collagen, type IV, alpha 4; collagen, type IV, alpha 5; collagen, type IV, alpha 6; dA149D17.3; dA24A23.1; dJ889N15.4 (Collagen Alpha 6(IV)); Goodpasture antigen; tumstatin; Type IV Collagen
Gene Aliases: ASLN; ATS; BSVD; CA44; CA54; COL4A1; COL4A2; COL4A3; COL4A4; COL4A5; COL4A6; CXDELq22.3; DELXq22.3; DFNX6; ICH; POREN2; RATOR
UniProt ID: (Human) P02462, (Human) P08572, (Human) Q01955, (Human) P53420, (Human) P29400, (Human) Q14031
Entrez Gene ID: (Human) 1282, (Human) 1284, (Human) 1285, (Human) 1286, (Human) 1287, (Human) 1288
extracellular matrix structural constituent
protein binding
extracellular matrix constituent conferring elasticity
platelet-derived growth factor binding
integrin binding
structural molecule activity
metalloendopeptidase inhibitor activity
extracellular matrix structural protein
extracellular matrix protein
patterning of blood vessels
brain development
neuromuscular junction development
extracellular matrix organization
collagen catabolic process
epithelial cell differentiation
blood vessel morphogenesis
retinal blood vessel morphogenesis
renal tubule morphogenesis
cellular response to amino acid stimulus
basement membrane organization
activation of cysteine-type endopeptidase activity involved in apoptotic process
cell adhesion
cell surface receptor signaling pathway
sensory perception of sound
blood circulation
cell proliferation
negative regulation of cell proliferation
negative regulation of endopeptidase activity
negative regulation of angiogenesis
glomerular basement membrane development
endothelial cell apoptotic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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