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Invitrogen
Collagen IV Recombinant Rabbit Monoclonal Antibody (3V3N3)
FIGURE: 1 / 2
Collagen IV Antibody (MA5-55419) in WB
Western blot analysis of lysates from HeLa cells, using Collagen IV Recombinant Monoclonal Antibody (Product # MA5-55419) at 1:1,000 dilution followed by a HRP-conjugated Goat anti-Rabbit IgG (H+L) secondary antibody at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Product Details
MA5-55419
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
3V3N3
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1573-1690 of human COL4A4.
Conjugate
Form
Liquid
Concentration
1.12 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.05% ProClin 300
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Dry ice (international)
Product Specific Information
Immunogen Sequence: AQAVAVHSQD QSIPPCPQTW RSLWIGYSFL MHTGAGDQGG GQALMSPGSC LEDFRAAPFL ECQGRQGTCH FFANKYSFWL TTVKADLQFS SAPAPDTLKE SQAQRQKISR CQVCVKYS
Target Information
Collagen IV is a type of collagen protein that is a major component of the basement membrane in various tissues, including the kidneys, lungs, and skin. It forms a network-like structure that provides support and stability to the surrounding tissue. Collagen IV is composed of three alpha chains, which are encoded by the COL4A1, COL4A2, and COL4A3 genes. Mutations in these genes can lead to various genetic disorders, such as Alport syndrome and Goodpasture syndrome, which affect the kidneys and lungs, respectively.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: alpha 1 type IV collagen; alpha-2 collagen type IV; alpha1(IV) collagen; arresten; canstatin; COL4A1 NC1 domain; col4a3; col4a4; Collagen 4; Collagen alpha-1(IV) chain; Collagen alpha-2(IV) chain; Collagen alpha-3(IV) chain; Collagen alpha-4(IV) chain; Collagen alpha-5(IV) chain; Collagen alpha-6(IV) chain; collagen IV, alpha-1 polypeptide; collagen IV, alpha-3 polypeptide; Collagen IV, alpha-4 polypeptide; collagen IV, alpha-5 polypeptide; collagen IV, alpha-6 polypeptide; collagen of basement membrane, alpha-1 chain; collagen of basement membrane, alpha-4 chain; collagen of basement membrane, alpha-5 chain; collagen of basement membrane, alpha-6; collagen type IV alpha3 chain; collagen type IV alpha5 chain; collagen, type IV, alpha 1; collagen, type IV, alpha 3 (Goodpasture antigen); collagen, type IV, alpha 4; collagen, type IV, alpha 5; collagen, type IV, alpha 6; dA149D17.3; dA24A23.1; Del(8)Bru44H; dJ889N15.4 (Collagen Alpha 6(IV)); Goodpasture antigen; procollagen type IV alpha 5; procollagen type IV alpha 6; procollagen, type IV, alpha 1; procollagen, type IV, alpha 2; procollagen, type IV, alpha 3; procollagen, type IV, alpha 4; procollagen, type IV, alpha 5; procollagen, type IV, alpha 6; retinal anterior wiring; tumstatin; Type IV Collagen
Gene Aliases: [a]3(IV); [a]4(IV); alpha3(IV); ASLN; ATS; BB116301; Bru; BSVD; CA44; CA54; Col4a-1; Col4a-2; COL4A1; COL4A2; COL4A3; COL4A4; COL4A5; COL4A6; CXDELq22.3; Del(8)44H; DELXq22.3; DFNX6; E130010M05Rik; ICH; POREN2; RATOR; Raw; RGD1565499; Svc
extracellular matrix structural constituent
protein binding
extracellular matrix constituent conferring elasticity
platelet-derived growth factor binding
integrin binding
structural molecule activity
metalloendopeptidase inhibitor activity
molecular_function
extracellular matrix structural protein
extracellular matrix protein
patterning of blood vessels
brain development
neuromuscular junction development
extracellular matrix organization
collagen catabolic process
epithelial cell differentiation
blood vessel morphogenesis
retinal blood vessel morphogenesis
renal tubule morphogenesis
cellular response to amino acid stimulus
basement membrane organization
activation of cysteine-type endopeptidase activity involved in apoptotic process
cell adhesion
cell surface receptor signaling pathway
sensory perception of sound
blood circulation
cell proliferation
negative regulation of cell proliferation
negative regulation of endopeptidase activity
negative regulation of angiogenesis
glomerular basement membrane development
endothelial cell apoptotic process
angiogenesis
transcription, DNA-templated
endodermal cell differentiation
cellular response to transforming growth factor beta stimulus
response to glucose
biological_process
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