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Invitrogen
CrkL Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 7
CrkL Antibody (PA5-88241) in ICC/IF
Immunofluorescence analysis of Crk-like protein was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CrkL Polyclonal Antibody (Product # PA5-88241) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conj... View More
Product Details
PA5-88241
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-303 of human CRKL (NP_0051981)
Conjugate
Form
Liquid
Concentration
1.22 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2804768
Product Specific Information
Immunogen sequence: MSSARFDSSD RSAWYMGPVS RQEAQTRLQG QRHGMFLVRD SSTCPGDYVL SVSENSRVSH YIINSLPNRR FKIGDQEFDH LPALLEFYKI HYLDTTTLIE PAPRYPSPPM GSVSAPNLPT AEDNLEYVRT LYDFPGNDAE DLPFKKGEIL VIIEKPEEQW WSARNKDGRV GMIPVPYVEK LVRSSPHGKH GNRNSNSYGI PEPAHAYAQP QTTTPLPAVS GSPGAAITPL PSTQNGPVFA KAIQKRVPCA YDKTALALEV GDIVKVTRMN INGQWEGEVN GRKGLFPFTH VKIFDPQNPD ENE; Positive Samples: LO2, Jurkat, SKOV3, Mouse brain, Rat heart
Target Information
May mediate the transduction of intracellular signals.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Crk-like protein; crk-like protein {ECO:0000250|UniProtKB:P46109}; crkl {ECO:0000312|EMBL:AAH85865.1, ECO:0000312|RGD:1308531}; OTTHUMP00000198539; v-crk avian sarcoma virus CT10 oncogene homolog-like; v-crk sarcoma virus CT10 oncogene homolog-like
Gene Aliases: 1110025F07Rik; AA589403; AI325100; CRKL; Crkol
UniProt ID: (Human) P46109, (Mouse) P47941, (Rat) Q5U2U2
Entrez Gene ID: (Human) 1399, (Mouse) 12929, (Rat) 287942
activation of MAPKK activity
blood vessel development
JNK cascade
Ras protein signal transduction
heart development
positive regulation of cell proliferation
organ morphogenesis
anterior/posterior pattern specification
intracellular signal transduction
thymus development
parathyroid gland development
cell-cell adhesion
positive regulation of substrate adhesion-dependent cell spreading
pattern specification process
biological_process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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