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Invitrogen
CrkL Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 5
CrkL Antibody (PA5-95058) in ICC/IF
Immunofluorescence analysis of Crk-like protein was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CrkL Polyclonal Antibody (Product # PA5-95058) at 5 µg in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjuga... View More
Product Details
PA5-95058
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human CrkL (286-303aa FPFTHVKIFDPQNPDENE).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide, 0.05mg thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2806864
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
May mediate the transduction of intracellular signals.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Crk-like protein; crk-like protein {ECO:0000250|UniProtKB:P46109}; crkl {ECO:0000312|EMBL:AAH85865.1, ECO:0000312|RGD:1308531}; OTTHUMP00000198539; v-crk avian sarcoma virus CT10 oncogene homolog-like; v-crk sarcoma virus CT10 oncogene homolog-like
Gene Aliases: 1110025F07Rik; AA589403; AI325100; CRKL; Crkol
UniProt ID: (Human) P46109, (Mouse) P47941, (Rat) Q5U2U2
Entrez Gene ID: (Human) 1399, (Mouse) 12929, (Rat) 287942
activation of MAPKK activity
blood vessel development
JNK cascade
Ras protein signal transduction
heart development
positive regulation of cell proliferation
organ morphogenesis
anterior/posterior pattern specification
intracellular signal transduction
thymus development
parathyroid gland development
cell-cell adhesion
positive regulation of substrate adhesion-dependent cell spreading
pattern specification process
biological_process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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