Proteintech

Cryptochrome 1 Polyclonal Antibody

Cryptochrome 1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 11

Cryptochrome 1 Antibody (13474-1-AP) in ICC/IF

Immunofluorescent analysis of Hela cells, using CRY1 antibody 13474-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Cryptochrome 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Cryptochrome 1 Antibody in Western Blot (WB)

Product Image Gallery

Product Details

13474-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:300-1:800

View 18 publications 18 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Cryptochrome 1 Fusion Protein Ag4270 (236-586 aa encoded by BC030519)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.19 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: RPRMNANSL LASPTGLSPY LRFGCLSCRL FYFKLTDLYK KVKKNSSPPL SLYGQLLWRE FFYTAATNNP RFDKMEGNPI CVQIPWDKNP EALAKWAEGR TGFPWIDAIM TQLRQEGWIH HLARHAVACF LTRGDLWISW EEGMKVFEEL LLDADWSINA GSWMWLSCSS FFQQFFHCYC PVGFGRRTDP NGDYIRRYLP VLRGFPAKYI YDPWNAPEGI QKVAKCLIGV NYPKPMVNHA EASRLNIERM KQIYQQLSRY RGLGLLASVP SNPNGNGGFM GYSAENIPGC SSSGSCSQGS GILHYAHGDS QQTHLLKQGR SSMGTGLSGG KRPSQEEDTQ SIGPKVQRQS TN (236-586 aa encoded by BC030519)

Target Information

Various biochemical, physiological and behavioral processes display circadian rhythms controlled by an internal biological clock. The central "gears" driving this clock appear to be composed of an autoregulatory transcription/post translation-based feedback loop. Cryptochrome 1 (CRY1) and 2 (CRY2) are DNA-binding flavoproteins that bear some homology to blue-light receptors and photolyases. In Drosophila, CRY is a photoreceptor for the circadian clock where it binds to the clock component TIM in a light-dependent fashion and blocks its function. Mammalian CRY1 and CRY2 function via light-independent interactions with circadian genes CLOCK and BMAL1, as well as with PER1, PER2, and TIM. They seem to act as light-independent components of the circadian clock and likely regulate Per1 transcriptional cycling via interactions with both the activator and its feedback inhibitors. Mutant mice not expressing the Cry1 or Cry2 protein display accelerated and delayed periodicity of locomotor activity, respectively. It appears that the combination of both proteins working together is essential to synchronize the organism to circadian phases. A critical balance between Cry1 and Cry2 is required for proper clock function; in complete darkness, double-mutant mice present with instantaneous arrhythmicity, indicating the absence of an internal circadian clock.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Subtype-specific circadian clock dysregulation modulates breast cancer biology, invasiveness, and prognosis.

bioRxiv : the preprint server for biology

Hammarlund JA,Li SY,Wu G,Lian JW,Howell SJ,Clarke R,Adamson A,Gonçalves CF,Hogenesch JB,Meng QJ,Anafi RC

Fri May 19 00:00:00 EDT 2023

Maternal circadian disruption before pregnancy impairs the ovarian function of female offspring in mice.

The Science of the total environment

Guan Y,Xu M,Zhang Z,Liu C,Zhou J,Lin F,Fang J,Zhang Y,Yue Q,Zhen X,Yan G,Sun H,Liu W

Wed Mar 15 00:00:00 EDT 2023

Sleep Fragmentation, Electroencephalographic Slowing, and Circadian Disarray in a Mouse Model for Intensive Care Unit Delirium.

Anesthesia and analgesia

Dulko E,Jedrusiak M,Osuru HP,Atluri N,Illendula M,Davis EM,Beenhakker MP,Lunardi N

Sat Jul 01 00:00:00 EDT 2023

Interaction of Bmal1 and eIF2α/ATF4 pathway was involved in Shuxie compound alleviation of circadian rhythm disturbance-induced hepatic endoplasmic reticulum stress.

Journal of ethnopharmacology

Zhang M,Wu W,Huang C,Cai T,Wang M,Zhao N,Liu S,Yang S

Thu Aug 10 00:00:00 EDT 2023

Dark-light cycle disrupts bone metabolism and suppresses joint deterioration in osteoarthritic rats.

Arthritis research & therapy

Song X,Zhao M,Tang J,Ma T,Bai H,Wang X,Liu L,Li T,Xu X,Sheng X,Zhao B,Wang Y,Wang T,Guo Y,Zhang X,Gao L

Tue Jun 28 00:00:00 EDT 2022

Development of human cartilage circadian rhythm in a stem cell-chondrogenesis model.

Theranostics

Naven MA,Zeef LAH,Li S,Humphreys PA,Smith CA,Pathiranage D,Cain S,Woods S,Bates N,Au M,Wen C,Kimber SJ,Meng QJ

Wed Jun 08 00:00:00 EDT 2022

Melatonin regulates the immune response and improves Sjögren's syndrome-like symptoms in NOD/Ltj Mice.

Biochemical pharmacology

Liu Y,Weng X,Wei M,Yu S,Ding Y,Cheng B

Fri Jul 01 00:00:00 EDT 2022

Capsaicin Attenuates Oleic Acid-Induced Lipid Accumulation via the Regulation of Circadian Clock Genes in HepG2 Cells.

Journal of agricultural and food chemistry

Li R,Xiao J,Cao Y,Huang Q,Ho CT,Lu M

Wed Jan 26 00:00:00 EST 2022

Age-dependent effects of a high-fat diet combined with dietary advanced glycation end products on cognitive function and protection with voluntary exercise.

Food & function

Luo L,Li R,Wang G,Chen J,Chen L,Qin LQ,Yu Z,Wan Z

Wed Apr 20 00:00:00 EDT 2022

Acute hypoxia induced dysregulation of clock-controlled ovary functions.

Frontiers in physiology

Ding M,Lu Y,Huang X,Xing C,Hou S,Wang D,Zhang Y,Wang W,Zhang C,Zhang M,Meng F,Liu K,Liu G,Zhao J,Song L

Wed Jan 11 00:00:00 EST 2023

hPER3 promotes adipogenesis via hHSP90AA1-mediated inhibition of Notch1 pathway.

Cell death & disease

Wan X,Zhu L,Zhao L,Peng L,Xiong J,Yang W,Yuan J,Liang F,Zhang K,Chen K

Fri Mar 19 00:00:00 EDT 2021

Decreased expression of the clock gene Bmal1 is involved in the pathogenesis of temporal lobe epilepsy.

Molecular brain

Wu H,Liu Y,Liu L,Meng Q,Du C,Li K,Dong S,Zhang Y,Li H,Zhang H

Wed Jul 14 00:00:00 EDT 2021

Bromide impairs the circadian clock and glycolytic homeostasis via disruption of autophagy in rat H9C2 cardiomyocytes.

BMC molecular and cell biology

Jiang Y,Gu Y,Xu H,Tian X,Zhang X,Xu X,Yan W,Zhang X

Fri Jun 19 00:00:00 EDT 2020

Hydrogen peroxide modulates clock gene expression via PRX2-STAT3-REV-ERBα/β pathway.

Free radical biology & medicine

Ji G,Lv K,Chen H,Wang Y,Zhang Y,Li Y,Qu L

Sun Dec 01 00:00:00 EST 2019

Jiao-tai-wan Up-regulates Hypothalamic and Peripheral Circadian Clock Gene Cryptochrome and Activates PI3K/AKT Signaling in Partially Sleep-deprived Rats.

Current medical science

Huang WY,Zou X,Lu FE,Su H,Zhang C,Ren YL,Fang K,Xu LJ,Wang KF,Chen QJ,Dong H

Wed Aug 01 00:00:00 EDT 2018

Circadian networks in human embryonic stem cell-derived cardiomyocytes.

EMBO reports

Dierickx P,Vermunt MW,Muraro MJ,Creyghton MP,Doevendans PA,van Oudenaarden A,Geijsen N,Van Laake LW

Sat Jul 01 00:00:00 EDT 2017

Circadian networks in human embryonic stem cell-derived cardiomyocytes.

EMBO reports

Dierickx P,Vermunt MW,Muraro MJ,Creyghton MP,Doevendans PA,van Oudenaarden A,Geijsen N,Van Laake LW

Sat Jul 01 00:00:00 EDT 2017

Effect of Jiaotai Pill () on intestinal damage in partially sleep deprived rats.

Chinese journal of integrative medicine

Huang WY,Zou X,Lu FE,Zhang C,Ren YL,Xu LJ,Wang KF,Dong H

Fri Dec 01 00:00:00 EST 2017

SCA1⁺ Cells from the Heart Possess a Molecular Circadian Clock and Display Circadian Oscillations in Cellular Functions.

Stem cell reports

Du Pré BC,Demkes EJ,Feyen DAM,Dierickx P,Crnko S,Kok BJM,Sluijter JPG,Doevendans PA,Vos MA,Van Veen TAB,Van Laake LW

Tue Sep 12 00:00:00 EDT 2017

SCA1⁺ Cells from the Heart Possess a Molecular Circadian Clock and Display Circadian Oscillations in Cellular Functions.

Stem cell reports

Du Pré BC,Demkes EJ,Feyen DAM,Dierickx P,Crnko S,Kok BJM,Sluijter JPG,Doevendans PA,Vos MA,Van Veen TAB,Van Laake LW

Tue Sep 12 00:00:00 EDT 2017

Rhythmic expression of miR-27b-3p targets the clock gene Bmal1 at the posttranscriptional level in the mouse liver.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Zhang W,Wang P,Chen S,Zhang Z,Liang T,Liu C

Wed Jun 01 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: cryptochrome 1 (photolyase-like); Cryptochrome-1

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Gene Aliases: AU020726; AU021000; CRY1; PHLL1

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UniProt ID: (Human) Q16526, (Rat) Q32Q86, (Mouse) P97784

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Entrez Gene ID: (Human) 1407, (Rat) 299691, (Mouse) 12952

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Function(s)

nucleotide binding transcription factor activity, transcription factor binding core promoter sequence-specific DNA binding core promoter binding DNA binding double-stranded DNA binding deoxyribodipyrimidine photo-lyase activity DNA (6-4) photolyase activity protein binding transcription factor binding blue light photoreceptor activity protein kinase binding phosphatase binding nuclear hormone receptor binding histone deacetylase binding ubiquitin binding DNA photolyase activity photoreceptor activity kinase binding DNA photolyase

Process(es)

negative regulation of transcription from RNA polymerase II promoter gluconeogenesis transcription, DNA-templated DNA damage induced protein phosphorylation blue light signaling pathway protein-chromophore linkage lipid storage negative regulation of protein ubiquitination response to insulin circadian regulation of gene expression response to glucagon glucose homeostasis regulation of circadian rhythm negative regulation of circadian rhythm entrainment of circadian clock by photoperiod negative regulation of G-protein coupled receptor protein signaling pathway negative regulation of transcription, DNA-templated regulation of DNA damage checkpoint negative regulation of glucocorticoid receptor signaling pathway negative regulation of glucocorticoid secretion DNA repair signal transduction circadian rhythm regulation of transcription, DNA-templated rhythmic process response to stimulus

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Cryptochrome 1 Polyclonal Antibody

Cryptochrome 1 Antibody (13474-1-AP) in ICC/IF

Product Details

13474-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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