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NeoBiotechnologies
Crystallin Alpha B Monoclonal Antibody (CPTC-CRYAB-1)
Product Details
1410-MSM1-P1
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Mouse
/ IgG2c, kappa
Class
Monoclonal
Type
Antibody
Clone
CPTC-CRYAB-1
Immunogen
Recombinant human full-length CRYAB protein
Conjugate
Form
Liquid
Concentration
200 µg/mL
Purification
Protein A/G
Storage buffer
PBS, pH 7.4, with 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Immunohistochemistry (PFA fixed): incubate antibody for 30 min RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes.
Target Information
Lens proteins consist almost entirely of crystallins (about 95%). Crystallins are also found vertebrate skeletal muscle tissue. In the lens, their structural function is to assist in maintaining the proper refractive index of the lens. The mammalian lens contains 3 major classes of crystallins: alpha, beta, and gamma. Alpha-crystallin is the largest of the crystallins and is composed of 2 primary gene products-alpha-A and alpha-B. There are at least 5 different proteins comprising the beta-crystallins. The gamma-crystallins are monomeric, but there are at least 5 gamma crystallins identified in bovine and rat lens. Alpha-Crystallin comprises 40% of total lens protein composition. In addition to maintaining proper refractive index, it also functions in a chaperone like manner by preventing the formation of aggregates possibly leading to cataract formation. It is believed that the phosphorylated states of the alpha-crystallin occur in response to cellular stress and may serve a structural control function and play a role in protein maintenance. Alpha-B crystallin has been linked to Alexander's disease where it accumulates in brain cells of those afflicted.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Alpha(B)-crystallin; Alpha-crystallin B chain; CryAB antibody; crystallin, alpha B; epididymis secretory protein Li 101; Heat shock protein beta-5; Heat shock protein family B member 5; heat-shock 20 kD like-protein; HspB5; Renal carcinoma antigen NY-REN-27; Rosenthal fiber component
Gene Aliases: AACRYA; CMD1II; CRYA2; CRYAB; CTPP2; CTRCT16; HEL-S-101; HSPB5; MFM2
UniProt ID: (Human) P02511, (Rat) P23928
Entrez Gene ID: (Human) 1410, (Rat) 25420
response to hypoxia
lens development in camera-type eye
protein folding
muscle contraction
tubulin complex assembly
muscle organ development
aging
negative regulation of gene expression
regulation of cell death
negative regulation of cell growth
microtubule polymerization or depolymerization
response to estradiol
negative regulation of intracellular transport
response to hydrogen peroxide
negative regulation of apoptotic process
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
protein homooligomerization
stress-activated MAPK cascade
apoptotic process involved in morphogenesis
cellular response to gamma radiation
regulation of cellular response to heat
negative regulation of reactive oxygen species metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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