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Product Details
H00023002-M03
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Mouse
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
4H3
Immunogen
DAAM1 (NP_055807, 1 a.a. approximately 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Conjugate
Form
Liquid
Concentration
See Label
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Sequence of this protein is as follows: MAPRKRGGRG ISFIFCCFRN NDHPEITYRL RNDSNFALQT MEPALPMPPV EELDVMFSEL VDELDLTDKH REAMFALPAE KKWQIYCSKK KDQEENKGAT SWPEFYIDQL
Target Information
DAAM1 is a diaphanous-related formin first studied as a novel Disheveled binding protein and shown to be crucial for the planar cell polarity pathway in Xenopus. DAAM1, like other formins, directs nucleation and elongation of new actin filaments using its conserved formin-homology-2 (FH2) domain. It is a Rho-regulated formin implicated in neuronal development and regulation of gastrulation. It was identified as a binding protein of the Wnt receptor-associated protein Disheveled. It also binds the GTP-bound RhoA and increases its GTP-bound form population in cells. It exists in an autoinhibited state via intramolecular interactions between its amino-terminal GBD and carboxyl-terminal DAD domains. Structurally the protein includes a GTPase-binding domain (GBD), a diaphanous inhibitory domain (DID), and a coiled-coil region followed by the FH1, FH2 and C-terminal diaphanous auto-regulatory domain (DAD). In humans, the DAAM1 gene is mapped to chromosome 14q23.1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Disheveled-associated activator of morphogenesis 1
Gene Aliases: DAAM1; KIAA0666
UniProt ID: (Human) Q9Y4D1
Entrez Gene ID: (Human) 23002, (Rat) 314212
Performance Guarantee
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