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FIGURE: 1 / 2
DAO Antibody (MA5-51215) in WB
Western blot analysis of DAO in extracts of various cell lines. Samples were incubated with DAO Recombinant monoclonal antibody (Product # MA5-51215) using a dilution of 1:20,000 followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.
Product Details
MA5-51215
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
1G4U2
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DAO (NP_001908.3).
Conjugate
Form
Liquid
Concentration
0.51 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_3094313
Product Specific Information
Immunogen sequence: MRVVVIGAGV IGLSTALCIH ERYHSVLQPL DIKVYADRFT PLTTTDVAAG LWQPYLSDPN NPQEADWSQQ TFDYLLSHVH SPNAENLGLF LISGYNLFHE
Target Information
This gene encodes the peroxisomal enzyme D-amino acid oxidase. The enzyme is a flavoprotein which uses flavin adenine dinucleotide (FAD) as its prosthetic group. Its substrates include a wide variety of D-amino acids, but it is inactive on the naturally occurring L-amino acids. Its biological function is not known; it may act as a detoxifying agent which removes D-amino acids that accumulate during aging. In mice, it degrades D-serine, a co-agonist of the NMDA receptor. This gene may play a role in the pathophysiology of schizophrenia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AOC1; D-amino acid oxidase 1; D-amino-acid oxidase; DAAO; DAMOX
Gene Aliases: AI987963; DAAO; DAMOX; DAO; Dao-1; Dao1; OXDA
UniProt ID: (Human) P14920, (Rat) O35078, (Mouse) P18894
Entrez Gene ID: (Human) 1610, (Rat) 114027, (Mouse) 13142
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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