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FIGURE: 1 / 1
DAPK1 Antibody (MA5-55263) in WB
Western Blot analysis of various lysates using DAPK1 Recombinant Monoclonal Antibody (Product # MA5-55263) at 1:1,000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3 min.
Product Details
MA5-55263
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
3E10G3
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 250-350 of human DAP Kinase 1 (DAPK1).
Conjugate
Form
Liquid
Concentration
1.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Dry ice (international)
Product Specific Information
Immunogen Sequence: DFIRRLLVKD PKKRMTIQDS LQHPWIKPKD TQQALSRKAS AVNMEKFKKF AARKKWKQSV RLISLCQRLS RSFLSRSNMS VARSDDTLDE EDSFVMKAII H
Target Information
DAPK1 is a large, calmodulin-dependent kinase involved in gamma-interferon induced programmed cell death and is a potential tumor suppressor. DAPK1 is involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and authophagy. It regulates both type I apoptotic and type II autophagic cell death signal, depending on the cellular setting. Type I apoptotic cell death is caspase-dependent, type II authophagic cell death is caspase-independent. DAPK1 phosphorylates PIN1 resulting in inhibition of its catalytic activity, nuclear localization, and cellular function. It also phosphorylates TPM1, STX1A, PRKD1, BECN1, TSC2, RPS6, MYL9, and DAPK3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DAP kinase 1; Death-associated protein kinase 1; DKFZp781I035; OTTHUMP00000216193
Gene Aliases: D13Ucla1; DAP-Kinase; DAPK; DAPK1
UniProt ID: (Human) P53355, (Mouse) Q80YE7
Entrez Gene ID: (Human) 1612, (Mouse) 69635
protein kinase activity
protein serine/threonine kinase activity
calmodulin-dependent protein kinase activity
protein binding
calmodulin binding
ATP binding
GTP binding
syntaxin-1 binding
identical protein binding
nucleotide binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
protein phosphorylation
apoptotic process
extrinsic apoptotic signaling pathway via death domain receptors
regulation of autophagy
negative regulation of translation
intracellular signal transduction
regulation of apoptotic process
positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
protein autophosphorylation
cellular response to interferon-gamma
apoptotic signaling pathway
negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
regulation of N-methyl-D-aspartate selective glutamate receptor activity
regulation of translation
signal transduction
phosphorylation
negative regulation of apoptotic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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