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DCP1A Antibody (PA5-121093) in WB
Western blot analysis of DCP1A in extracts of various cell lines. Samples were incubated with DCP1A Polyclonal antibody (Product # PA5-121093) using a dilution of 1:500, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-121093
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 303-582 of human DCP1A (NP_060873.4).
Conjugate
Form
Liquid
Concentration
1.23 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2914665
Product Specific Information
Positive test controls include: HepG2, Jurkat, Mouse brain, Rat brain. The target is usually found in the following locations: Cytoplasm, Nucleus, P-body.
Immunogen sequence: NEKHAPTYTI PLSPVLSPTL PAEAPTAQVP PSLPRNSTMM QAVKTTPRQR SPLLNQPVPE LSHASLIANQ SPFRAPLNVT NTAGTSLPSV DLLQKLRLTP QHDQIQTQPL GKGAMVASFS PAAGQLATPE SFIEPPSKTA AARVAASASL SNMVLAPLQS MQQNQDPEVF VQPKVLSSAI PVAGAPLVTA TTTAVSSVLL APSVFQQTVT RSSDLERKAS SPSPLTIGTP ESQRKPSIIL SKSQLQDTLI HLIKNDSSFL STLHEVYLQV LTKNKDNHNL
Target Information
Signal transduction of TGF-beta superfamily members is regulated by Smad proteins. In particular, Smads influence specific gene transcription by relaying signals from the cell membrane to the nucleus. Smad4 plays an essential role in TGF-beta-induced transcriptional activation wherein phosphorylated receptor-associated Smads associate with Smad4. Furthermore, SMIF (Smad4-intereacting protein) and Smad4 complex with TGF-beta and BMP4. An increase in Smad4 concentration increases the translocation of this complex to the nucleus. SMIF and Smad4 interact directly through a EVH1/WH1 domain on SMIF and a proline-rich activation domain on Smad4. Smad4 is essential to nuclear translocation of SMIF as deletion of the Smad4-interacting domain (located in the N-terminal 100 amino acids) of SMIF eliminates TGF-beta-induced nuclear translocation of SMIF. The human SMIF gene is ubiquitously expressed and encodes a protein with a relative molecular mass of 70 kDa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DCP1 decapping enzyme homolog A; DCP1 decapping enzyme-like protein A; decapping enzyme; decapping enzyme hDcp1a; FLJ21691; MAD homolog 4 interacting transcription coactivator 1; MAD homolog 4-interacting transcription coactivator 1; mRNA-decapping enzyme 1A; putative protein product of Nbla00360; Smad4-interacting transcriptional co-activator; test2; Transcription factor SMIF
Gene Aliases: 1110066A22Rik; 4930568L04Rik; AU019772; D14Ertd817e; DCP1A; HSA275986; Mitc1; Nbla00360; RGD1561298; SMAD4IP1; SMIF
UniProt ID: (Human) Q9NPI6, (Mouse) Q91YD3
Entrez Gene ID: (Human) 55802, (Rat) 361109, (Mouse) 75901
mRNA binding
protein binding
enzyme activator activity
hydrolase activity
enzyme regulator activity
identical protein binding
RNA 7-methylguanosine cap binding
GPI-anchor transamidase activity
transcription factor binding
coenzyme F390-A hydrolase activity
coenzyme F390-G hydrolase activity
mRNA capping factor
nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
deadenylation-dependent decapping of nuclear-transcribed mRNA
deadenylation-independent decapping of nuclear-transcribed mRNA
positive regulation of catalytic activity
regulation of mRNA stability
exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay
protein localization to cytoplasmic stress granule
regulation of catalytic activity
transforming growth factor beta receptor signaling pathway
positive regulation of transcription, DNA-templated
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