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Invitrogen
DCP1A Recombinant Rabbit Monoclonal Antibody (JB51-34)
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 11
DCP1A Antibody (MA5-34669) in ICC/IF
Immunofluorescence analysis of Dcp1a was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for overnight at room temperature. The cells were labeled with DCP1A Recombinant Rabbit Monoclonal Antibody (JB51-34) (Product # MA5-34669) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fl... View More
Product Details
MA5-34669
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JB51-34
Immunogen
Recombinant protein within Human Dcp1a aa 460-582
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles, store in dark
Shipping conditions
Wet ice
RRID
AB_2848577
Product Specific Information
Positive Control: LOVO, SH-SY-5Y, SiHa, human liver tissue, human placenta tissue.
Target Information
Signal transduction of TGF-beta superfamily members is regulated by Smad proteins. In particular, Smads influence specific gene transcription by relaying signals from the cell membrane to the nucleus. Smad4 plays an essential role in TGF-beta-induced transcriptional activation wherein phosphorylated receptor-associated Smads associate with Smad4. Furthermore, SMIF (Smad4-intereacting protein) and Smad4 complex with TGF-beta and BMP4. An increase in Smad4 concentration increases the translocation of this complex to the nucleus. SMIF and Smad4 interact directly through a EVH1/WH1 domain on SMIF and a proline-rich activation domain on Smad4. Smad4 is essential to nuclear translocation of SMIF as deletion of the Smad4-interacting domain (located in the N-terminal 100 amino acids) of SMIF eliminates TGF-beta-induced nuclear translocation of SMIF. The human SMIF gene is ubiquitously expressed and encodes a protein with a relative molecular mass of 70 kDa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DCP1 decapping enzyme homolog A; DCP1 decapping enzyme-like protein A; decapping enzyme; decapping enzyme hDcp1a; FLJ21691; MAD homolog 4 interacting transcription coactivator 1; MAD homolog 4-interacting transcription coactivator 1; mRNA-decapping enzyme 1A; putative protein product of Nbla00360; Smad4-interacting transcriptional co-activator; Transcription factor SMIF
Gene Aliases: 1110066A22Rik; 4930568L04Rik; AU019772; D14Ertd817e; DCP1A; HSA275986; Mitc1; Nbla00360; SMAD4IP1; SMIF
UniProt ID: (Human) Q9NPI6, (Mouse) Q91YD3
Entrez Gene ID: (Human) 55802, (Mouse) 75901
nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
deadenylation-dependent decapping of nuclear-transcribed mRNA
deadenylation-independent decapping of nuclear-transcribed mRNA
positive regulation of catalytic activity
regulation of mRNA stability
exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay
protein localization to cytoplasmic stress granule
transforming growth factor beta receptor signaling pathway
positive regulation of transcription, DNA-templated
regulation of catalytic activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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