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DHX8 Antibody (A300-624A-T) in WB
Detection of human DHX8 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DHX8 antibody A300-624A-M (lot A300-624A-M-2) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.
Product Details
A300-624A-T
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Between 325 and 375
Conjugate
Form
Liquid
Concentration
200 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
This gene is a member of the DEAH box polypeptide family. The encoded protein contains the DEAH (Asp-Glu-Ala-His) motif which is characteristic of all DEAH box proteins, and is thought to function as an ATP-dependent RNA helicase that regulates the release of spliced mRNAs from spliceosomes prior to their export from the nucleus. This protein may be required for the replication of human immunodeficiency virus type 1 (HIV-1). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014].
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP-dependent RNA helicase DHX8; DEAD (aspartate-glutamate-alanine-aspartate) box polypeptide 8; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 8 (RNA helicase); DEAH (Asp-Glu-Ala-His) box polypeptide 8; DEAH box protein 8; RNA helicase; RNA helicase HRH1
Gene Aliases: DDX8; DHX8; HRH1; mDEAH6; mKIAA4096; PRP22; PRPF22
UniProt ID: (Human) Q14562, (Mouse) A2A4P0
Entrez Gene ID: (Human) 1659, (Mouse) 217207
Performance Guarantee
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