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DOK1 Antibody (MA5-36191) in WB
Western blot analysis of DOK1 using a DOK1 monoclonal antibody (Product #MA5-36191). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody at a dilution of 1:500 was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: K562 cell lysate, Lane 2: Rat skin tissue lysate
Product Details
MA5-36191
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JE54-52
Immunogen
Recombinant protein within Human DOK1 aa 360-481
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 0.05% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2890524
Target Information
DOK1 (P62dok, Docking protein 1) is believed to be a mainly cytoplasmic adaptor protein which down-regulates mitogen-activated protein kinase activation, inhibits cell proliferation and transformation, and promotes cell spreading and cell migration. DOK1 is a major substrate for many tyrosine kinases including c-kit, v-abl, vFPS, EGF, and PDGF. Upon phosphorylation by kinases, DOK1 forms a complex with ras GTPase-activating protein. Phosphorylation on tyrosine residues by the insulin receptor kinase results in the negative regulation of the insulin signaling pathway. DOK1 contains a putative pleckstrin homology domain at the amino terminus and ten PXXP SH3 recognition motifs.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Docking protein 1; docking protein 1, 62kDa (downstream of tyrosine kinase 1); DOK 1; Downstream of tyrosine kinase 1; downstream of tyrosine kinase-1; MGC117395; MGC138860; p62 dok; p62(dok); pp62; TLP1; TP1; TROVE domain family; TROVE1; VAULT2
Gene Aliases: Dok-1; DOK1; P62DOK; pp62
UniProt ID: (Human) Q9UHG6, (Rat) Q4QQV2
Entrez Gene ID: (Human) 1796, (Rat) 312477
Performance Guarantee
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