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Invitrogen
DRD1 Recombinant Rabbit Monoclonal Antibody (1H8L2)
FIGURE: 1 / 5
DRD1 Antibody (702593) in ICC/IF
For immunocytochemistry analysis, HEK 293 cells stably transfected with Dopamine D1 receptor (D1R) were grown on poly-L-lysine-coated coverslips overnight. Cells were then treated with 10 µM dopamine for 30 min, washed with PBS and fixed with 4% paraformaldehyde and 0.2% picric acid in phosphate buffer (pH 6.9) for 30 min at room temperature and washed several times with phosphate buffer. Specimens were permeabilized with ice-cold methanol and then incubated with Anti- DRD1 Recombinant Rabbit Monoclonal Antibody (Product # 702593, 1 µg/mL)... View More
Product Details
702593
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Published species
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
1H8L2
Immunogen
Peptide corresponding to human Dopamine Receptor D1 [aa427-aa446]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2734804
Product Specific Information
This antibody is predicted to react with Monkey, Cat, Bovine.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
The D1 subtype is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. Alternate transcription initiation sites result in two transcript variants of this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: D(1A) dopamine receptor; D1a receptor; Dopamine 1 receptor; Dopamine D1 receptor; dopamine receptor D1A; Dopamine-1A receptor
Gene Aliases: D1a; DADR; Drd-1; DRD1; DRD1A
UniProt ID: (Human) P21728
Entrez Gene ID: (Human) 1812, (Rat) 24316
dopamine neurotransmitter receptor activity, coupled via Gs
G-protein alpha-subunit binding
dopamine neurotransmitter receptor activity
protein binding
drug binding
protein phosphatase binding
angiotensin receptor binding
D3 dopamine receptor binding
protein complex binding
dopamine binding
protein heterodimerization activity
ATPase binding
G-protein coupled receptor activity
receptor binding
G-protein coupled receptor
temperature homeostasis
conditioned taste aversion
behavioral fear response
synaptic transmission, dopaminergic
response to amphetamine
negative regulation of protein kinase activity
protein import into nucleus
G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger
adenylate cyclase-activating G-protein coupled receptor signaling pathway
activation of adenylate cyclase activity
adenylate cyclase-activating dopamine receptor signaling pathway
synapse assembly
learning
memory
mating behavior
grooming behavior
adult walking behavior
visual learning
positive regulation of adenylate cyclase activity involved in G-protein coupled receptor signaling pathway
astrocyte development
response to activity
dopamine transport
transmission of nerve impulse
neuronal action potential
regulation of vasoconstriction
calcium-mediated signaling
dentate gyrus development
striatum development
orbitofrontal cortex development
cerebral cortex GABAergic interneuron migration
positive regulation of cell migration
negative regulation of cell migration
peristalsis
positive regulation of cAMP biosynthetic process
response to food
response to estradiol
response to retinoic acid
cellular response to insulin stimulus
response to nicotine
operant conditioning
social behavior
response to antidepressant
regulation of dopamine metabolic process
vasodilation
negative regulation of circadian sleep/wake cycle, sleep
dopamine metabolic process
response to drug
maternal behavior
response to amino acid
positive regulation of potassium ion transport
response to morphine
response to ethanol
positive regulation of membrane potential
glucose import
habituation
sensitization
behavioral response to cocaine
regulation of long-term neuronal synaptic plasticity
response to steroid hormone
positive regulation of release of sequestered calcium ion into cytosol
positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway
regulation of dopamine uptake involved in synaptic transmission
positive regulation of synaptic transmission, glutamatergic
prepulse inhibition
phospholipase C-activating dopamine receptor signaling pathway
long-term synaptic potentiation
long term synaptic depression
cellular response to hypoxia
cellular response to catecholamine stimulus
positive regulation of long-term synaptic potentiation
positive regulation of feeding behavior
startle response
intracellular protein transport
muscle contraction
G-protein coupled receptor signaling pathway
dopamine receptor signaling pathway
chemical synaptic transmission
locomotory behavior
feeding behavior
associative learning
response to organonitrogen compound
response to organic cyclic compound
hippocampus development
phosphatidylinositol catabolic process
response to cocaine
regulation of ion transport
positive regulation of adenylate cyclase activity
phosphatidylinositol metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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