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DUSP3 Antibody (TA812964) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DUSP3 (RC201119, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-DUSP3. Positive lysates LY401320 (100 µg) and LC401320 (20 µg) can be purchased separately from OriGene.
Product Details
TA812964
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
OTI2B12
Immunogen
Full length human recombinant protein of human DUSP3 produced in E.coli.
Conjugate
Form
Liquid
Concentration
0.5-1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 1% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
Protein tyrosine phosphatases (PTPs) are a group of enzymes that remove phosphate groups from phosphorylated tyrosine residues on proteins. Together with tyrosine kinases, PTPs regulate the phosphorylation state of many important signaling molecules, such as the MAP kinase family. Recently, increasing attention has been focused on the growing family of PTPs. Like PTKs, PTPs have been implicated in cell signaling, cell growth and proliferation, and oncogenic transformation. Moreover, some PTPs can be involved in cell cycle regulation and embryogenesis. Dual specificity phosphatases (DSPs) are an emerging subclass of the protein tyrosine phosphatase (PTP) gene superfamily, which appears to be selective for dephosphorylating the critical phosphothreonine and phosphotyrosine residues. The prototypical DSP is the VH1 gene in vaccinia virus expressed in late-stage viral infection. A shallow active site pocket in VHR allows for the hydrolysis of phosphorylated serine, threonine, or tyrosine protein residues, whereas the deeper active site of protein tyrosine phosphatases (PTPs) restricts substrate specificity to only phosphotyrosine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Dual specificity protein phosphatase 3; Dual specificity protein phosphatase VHR; DUSP 3; DUSP-3; serine/threonine specific protein phosphatase; Vaccinia H1-related phosphatase; vaccinia virus phosphatase VH1-related; VHR
Gene Aliases: DUSP3; VHR
UniProt ID: (Human) P51452
Entrez Gene ID: (Human) 1845
inactivation of MAPK activity
dephosphorylation
peptidyl-tyrosine dephosphorylation
negative regulation of MAPK cascade
positive regulation of mitotic cell cycle
negative regulation of JNK cascade
negative regulation of T cell receptor signaling pathway
negative regulation of T cell activation
negative regulation of ERK1 and ERK2 cascade
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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