Invitrogen

Dicer Monoclonal Antibody (N167/7), FITC

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Product Details

MA5-45569

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunocytochemistry (ICC/IF)

1:100
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

N167/7

Immunogen

Fusion protein amino acids 1638-1899 of mouse Endoribonuclease Dicer
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Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

9.1mM sodium bicarbonate/PBS, pH 7.4, with 581mM DMSO, 123mM ethanolamine

Contains

no preservative

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2932023

Product Specific Information

1 µg/mL of MA5-45569 was sufficient for detection of Dicer in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |Detects approximately 215kDa.

This antibody was formerly sold as clone S167-7.

Target Information

Dicer is a double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. It cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a means to specifically regulate the expression of target genes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DCR; DCR-1; dicer 1 ribonuclease III; dicer 1, double-stranded RNA-specific endoribonuclease; dicer 1, ribonuclease type III; Dicer1, Dcr-1 homolog; double-strand-specific ribonuclease mDCR-1; Endoribonuclease Dicer; Helicase MOI; Helicase with RNase motif; helicase-moi

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Gene Aliases: 1110006F08Rik; D12Ertd7e; DCR1; DICER; DICER1; Dicer1e; HERNA; K12H4.8-LIKE; KIAA0928; mKIAA0928; MNG1; RMSE2

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UniProt ID: (Human) Q9UPY3

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Entrez Gene ID: (Human) 23405, (Mouse) 192119, (Rat) 299284

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Function(s)
double-stranded RNA binding helicase activity endoribonuclease activity ribonuclease III activity deoxyribonuclease I activity protein binding ATP binding protein domain specific binding siRNA binding metal ion binding pre-miRNA binding nucleotide binding nucleic acid binding RNA binding nuclease activity endonuclease activity hydrolase activity endoribonuclease activity, producing 5'-phosphomonoesters miRNA binding molecular_function
Process(es)
negative regulation of transcription from RNA polymerase II promoter meiotic spindle organization angiogenesis regulation of protein phosphorylation hair follicle development apoptotic DNA fragmentation RNA processing cell proliferation regulation of Notch signaling pathway post-embryonic development zygote asymmetric cell division regulation of gene expression negative regulation of Schwann cell proliferation positive regulation of gene expression regulation of muscle cell apoptotic process positive regulation of Schwann cell differentiation myoblast differentiation involved in skeletal muscle regeneration rRNA catabolic process stem cell population maintenance spinal cord motor neuron differentiation nerve development olfactory bulb interneuron differentiation cerebral cortex development lung development embryonic limb morphogenesis production of siRNA involved in RNA interference targeting of mRNA for destruction involved in RNA interference gene silencing by RNA pre-miRNA processing hair follicle morphogenesis regulation of myelination positive regulation of myelination peripheral nervous system myelin formation conversion of ds siRNA to ss siRNA involved in RNA interference siRNA loading onto RISC involved in RNA interference embryonic hindlimb morphogenesis production of miRNAs involved in gene silencing by miRNA multicellular organism growth miRNA loading onto RISC involved in gene silencing by miRNA conversion of ds siRNA to ss siRNA regulation of odontogenesis of dentin-containing tooth regulation of viral genome replication regulation of cell differentiation regulation of neuron differentiation positive regulation of transcription from RNA polymerase II promoter mRNA stabilization spleen development digestive tract development reproductive structure development regulation of oligodendrocyte differentiation epidermis morphogenesis branching morphogenesis of an epithelial tube neuron projection morphogenesis anatomical structure development regulation of neurogenesis cartilage development spindle assembly regulation of RNA metabolic process defense response to virus regulation of cell cycle cardiac muscle cell development inner ear receptor cell development negative regulation of glial cell proliferation intestinal epithelial cell development cardiac neural crest cell development involved in outflow tract morphogenesis ganglion development regulation of enamel mineralization hair follicle cell proliferation RNA phosphodiester bond hydrolysis RNA phosphodiester bond hydrolysis, endonucleolytic regulation of miRNA metabolic process positive regulation of miRNA metabolic process biological_process
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