Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
Drosha Monoclonal Antibody (S.12.0)
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 4
Drosha Antibody (MA5-14784) in WB
Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of HeLa (Lane 1), HEK-293 (Lane 2), C2C12 (Lane 3) and K-562 (Lane 4). The blot was probed with anti-Drosha Monoclonal Antibody (Product # MA5-14784, 1:1000 dilution) and detected by chemiluminescence using Goat anti Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 180 kDa band corresponding to Drosha was detected across the cell lines tested.
Product Details
MA5-14784
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Monoclonal
Type
Antibody
Clone
S.12.0
Immunogen
Synthetic peptide corresponding to residues surrounding His549 of human Drosha
Conjugate
Form
Liquid
Concentration
615 µg/mL
Purification
Affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
<0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_10983204
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
Members of the ribonuclease III superfamily of double-stranded (ds) RNA-specific endoribonucleases participate in diverse RNA maturation and decay pathways in eukaryotic and prokaryotic cells (Fortin et al., 2002). The RNase III Drosha is the core nuclease that executes the initiation step of microRNA (miRNA) processing in the nucleus (Lee et al., 2003).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: drosha, double-stranded RNA-specific endoribonuclease; drosha, ribonuclease type III; ethanol induced 2; MGC115770; nuclear RNase III Drosha; OTTHUMP00000220910; OTTHUMP00000220911; OTTHUMP00000220912; OTTHUMP00000220913; p241; Protein Drosha; putative protein p241 which interacts with transcription factor Sp1; putative ribonuclease III; Ribonuclease 3; Ribonuclease III; ribonuclease III, nuclear; ribonuclease type III, nuclear; RNase III
Gene Aliases: 1110013A17Rik; AI874853; DROSHA; ETOHI2; HSA242976; RANSE3L; RN3; RNASE3L; RNASEN
UniProt ID: (Human) Q9NRR4, (Mouse) Q5HZJ0
Entrez Gene ID: (Human) 29102, (Mouse) 14000
lipopolysaccharide binding
double-stranded RNA binding
ribonuclease III activity
protein binding
protein homodimerization activity
poly(A) RNA binding
metal ion binding
primary miRNA binding
RNA binding
nuclease activity
endonuclease activity
endoribonuclease activity
hydrolase activity
endoribonuclease
miRNA metabolic process
positive regulation of gene expression
rRNA catabolic process
production of siRNA involved in RNA interference
gene silencing by RNA
primary miRNA processing
pre-miRNA processing
ribosome biogenesis
defense response to Gram-negative bacterium
defense response to Gram-positive bacterium
RNA phosphodiester bond hydrolysis, endonucleolytic
regulation of miRNA metabolic process
RNA processing
regulation of gene expression
RNA phosphodiester bond hydrolysis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support