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Dynein Antibody (PA5-89506) in WB
Western blot analysis of Dynein in extracts of various cell lines. Samples were incubated with Dynein Polyclonal antibody (Product # PA5-89506) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Product Details
PA5-89506
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-89 of human DYNLL2 (NP_5424081)
Conjugate
Form
Liquid
Concentration
0.18 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2805618
Product Specific Information
Immunogen sequence: MSDRKAVIKN ADMSEDMQQD AVDCATQAME KYNIEKDIAA YIKKEFDKKY NPTWHCIVGR NFGSYVTHET KHFIYFYLGQ VAILLFKSG; Positive Samples: Mouse brain, Mouse kideny, Rat brain; Cellular Location: Cytoplasm, cytoskeleton
Target Information
The directed movement of membrane-bounded organelles is an important process for various cellular functions including membrane transport, secretion, and axonal transport. Cytoplasmic dynein, a ubiquitous minus end-directed microtubule-based motor protein, is believed to be responsible for the retrograde transport of membranous organelles from the synapse to the cell body in fast axonal transport. The cytoplasmic dynein complex is composed of two ~530 kDa heavy chains, and intermediate chains of ~74 kDa and ~53-59 kDa.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 8 kDa dynein light chain; 8 kDa dynein light chain b; 8kD LC; 8kDa LC; cytoplasmic dynein light polypeptide; cytosolic DH IC-1; DLC8; DLC8b; dynein LC8; Dynein light chain 1, cytoplasmic; Dynein light chain 2, cytoplasmic; Dynein light chain LC8-type 1; Dynein light chain LC8-type 2; dynein light chain-2; dynein, cytoplasmic, light chain 1; dynein, cytoplasmic, light peptide; dynein, cytoplasmic, light polypeptide 1; dynein, light chain, LC8-type 1; dynein, light chain, LC8-type 2; mPIN; PIN; Protein inhibitor of neuronal nitric oxide synthase; protein inhibitor of nitric oxide synthase; radial spoke 22 homolog
Gene Aliases: 1700064A15Rik; 6720463E02Rik; 8kDLC; C87222; DLC1; DLC2; DLC8; DLC8b; DNCL1; DNCL1B; DNCLC1; DYNLL1; DYNLL2; HDLC1; LC8; LC8a; PIN; RSPH22
UniProt ID: (Human) P63167, (Human) Q96FJ2, (Rat) P63170, (Mouse) P63168, (Rat) Q78P75, (Mouse) Q9D0M5
Entrez Gene ID: (Human) 8655, (Human) 140735, (Rat) 58945, (Mouse) 56455, (Rat) 140734, (Mouse) 68097
G2/M transition of mitotic cell cycle
transcription, DNA-templated
regulation of transcription, DNA-templated
ER to Golgi vesicle-mediated transport
apoptotic process
female gamete generation
anatomical structure morphogenesis
viral process
macroautophagy
antigen processing and presentation of exogenous peptide antigen via MHC class II
substantia nigra development
actin cytoskeleton organization
intraciliary retrograde transport
negative regulation of phosphorylation
cilium morphogenesis
positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway
microtubule-based process
synaptic target recognition
nitric oxide biosynthetic process
transport
neurotransmitter metabolic process
negative regulation of catalytic activity
regulation of catalytic activity
microtubule-based movement
negative regulation of nitric oxide biosynthetic process
metabolic process
intracellular transport
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