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E2-25K Antibody (A304-125A) in WB
Detection of human and mouse E2-25K by western blot. Samples: Whole cell lysate (50 µg) from 293T, HeLa, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-E2-25K antibody A304-125A (lot A304-125A-1) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Product Details
A304-125A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 150 to 200 of human Ubiquitin-Conjugating Enzyme E2K.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Bovine
Target Information
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of 'Lys-48'-linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated substrate protein. Mediates the selective degradation of short-lived and abnormal proteins, such as the endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequence degradation of p53/TP53. Proposed to be involved in ubiquitination and proteolytic processing of NF-kappa-B; in vitro supports ubiquitination of NFKB1. In case of infection by cytomegaloviruses may be involved in the US11-dependent degradation of MHC class I heavy chains following their export from the ER to the cytosol. In case of viral infections may be involved in the HPV E7 protein-dependent degradation of RB1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp564C1216; DKFZp686J24237; E2 ubiquitin-conjugating enzyme K; E2(25K); HIP-2; huntingtin interacting protein 2; Huntingtin-interacting protein 2; Ubiquitin carrier protein; ubiquitin conjugating enzyme E2K; Ubiquitin-conjugating enzyme E2 K; Ubiquitin-conjugating enzyme E2(25K); Ubiquitin-conjugating enzyme E2-25 kDa; ubiquitin-conjugating enzyme E2-25K; ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast); Ubiquitin-protein ligase
Gene Aliases: AW492011; D5Ertd601e; E2-25K; HIP2; HYPG; LIG; UBC1; UBE2K
UniProt ID: (Human) P61086, (Mouse) P61087
Entrez Gene ID: (Human) 3093, (Mouse) 53323
ubiquitin-dependent protein catabolic process
positive regulation of peptidyl-threonine phosphorylation
free ubiquitin chain polymerization
regulation of proteasomal ubiquitin-dependent protein catabolic process
cellular response to interferon-beta
proteasome-mediated ubiquitin-dependent protein catabolic process
positive regulation of type I interferon-mediated signaling pathway
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
protein K48-linked ubiquitination
protein polyubiquitination
protein ubiquitination
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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