Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 1
E2F8 Antibody (PA5-88117) in WB
Western blot analysis of E2F8 in extracts of various cell lines. Samples were incubated with E2F8 Polyclonal antibody (Product # PA5-88117) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.
Product Details
PA5-88117
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 788-867 of human E2F8 (NP_0789562)
Conjugate
Form
Liquid
Concentration
4.3 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2804664
Product Specific Information
Immunogen sequence: PVPGQSQPNG QSVAVTGAQQ PVPVTPKGSQ LVAESFFRTP GGPTKPTSSS CMDFEGANKT SLGTLFVPQR KLEVSTEDVH
Positive Samples: SW480, Mouse testis; Cellular Location: Nucleus
Target Information
E2F transcription factors, such as E2F8, are essential for orchestrating expression of genes required for cell cycle progression and proliferation (Christensen et al., 2005).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: E2F 8; E2F family member 8; E2F-8; FLJ23311; Transcription factor E2F8
Gene Aliases: 4432406C08Rik; AA410048; E2F-8; E2F8
UniProt ID: (Human) A0AVK6, (Mouse) Q58FA4
Entrez Gene ID: (Human) 79733, (Mouse) 108961
RNA polymerase II core promoter proximal region sequence-specific DNA binding
core promoter binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
transcription corepressor activity
protein binding
protein homodimerization activity
DNA binding
identical protein binding
RNA metabolism protein
nucleic acid metabolism protein
general transcription factor
negative regulation of transcription from RNA polymerase II promoter
placenta development
sprouting angiogenesis
transcription, DNA-templated
cell proliferation
negative regulation of cytokinesis
positive regulation of DNA endoreduplication
cell cycle comprising mitosis without cytokinesis
positive regulation of transcription from RNA polymerase II promoter
trophoblast giant cell differentiation
chorionic trophoblast cell differentiation
hepatocyte differentiation
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
cell cycle
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support