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Invitrogen
EB1 Monoclonal Antibody (KT51)
FIGURE: 1 / 1
EB1 Antibody (MA1-72531) in ICC/IF
Oncotarget 2021 -
Figure 9 Super-resolved images for EB1-mediated microtubule stabilization. The SIM images reveal clear localization of EB1 at the ends of microtubules in the control and EGCG treated cells. The EB1 localization was severely hampered and less abundant in MG treated cells. This effect of MG is inhibited in presence of EGCG. The rescued colocalization is visualized as yellow fluorescence.
Product Details
MA1-72531
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Hamster,
Human,
Mouse,
Non-human primate
Published species
Not Applicable
Host/Isotype
Rat
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
KT51
Immunogen
The C-terminal part of mouse EB1 (amino acids 125-268)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2235110
Product Specific Information
This antibody will not cross-react with mouse EB2 or human EB3.
Target Information
EB1 family proteins are evolutionarily conserved proteins that bind microtubule plus-ends and centrosomes and regulate the dynamics and organization of microtubules. The MAPRE genes encode the EB1 family proteins. The human MAPRE genes MAPRE1, MAPRE2, and MAPRE3 code for EB1, RP1, and EBF3 proteins, respectively. EB1 family members appear to be expressed ubiquitously. The EB1 (MAPRE1) gene, at 20q11. 2, is fused to MLL in an adult patient with pro-B acute lymphoblastic leukemia. EB1 is a microtubule-associated protein that interacts with the colorectal adenomatous polyposis coli tumor-suppressor protein and plays important roles in regulating microtubule dynamics, cell polarity, and chromosome stability.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: adenomatosis polyposis coli binding protein Eb1; adenomatous polyposis coli-binding protein EB1; APC-binding protein EB1; EB1; End-binding protein 1; MGC117374; MGC129946; Microtubule-associated protein RP/EB family member 1; microtubule-associated protein RP/EB family member 1-like protein; microtubule-associated protein, RP/EB family, member 1
Gene Aliases: 5530600P05Rik; AI462499; AI504412; AW260097; BIM1p; D2Ertd459e; EB1; MAPRE1
UniProt ID: (Human) Q15691, (Mouse) Q61166
Entrez Gene ID: (Human) 22919, (Mouse) 13589, (Chinese hamster) 100751441
G2/M transition of mitotic cell cycle
sister chromatid cohesion
mitotic nuclear division
cell proliferation
positive regulation of cell migration
negative regulation of microtubule polymerization
protein localization to microtubule
cell division
cell-cell adhesion
positive regulation of microtubule plus-end binding
negative regulation of microtubule binding
cell cycle
regulation of microtubule plus-end binding
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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