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ECSIT Antibody (PA5-115158) in IHC (P)
Immunohistochemistry analysis of ECSIT in mouse brain tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-115158) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.
Product Details
PA5-115158
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ECSIT(Accession Q9BQ95), corresponding to amino acid residues K251-Y301.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2899794
Product Specific Information
Antibody detects endogenous levels of total ECSIT.
Target Information
Activation of NF-kappa-B as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses. Signals from these receptors are relayed by a number of adapter molecules such as TRIF, TIRAP, and MyD88 to kinases such as IRAK and other intermediates such as TNF receptor associated factor (TRAF)-6. ECSIT (evolutionarily conserved signaling intermediate in Toll pathways) was initially identified as a cytoplasmic protein interacting specifically with TNF receptor associated factor (TRAF)-6 in the TLR pathway. Recently however, ECSIT has also been shown to be required for bone morphogenetic protein (Bmp) signaling and mesoderm formation during mouse embryogenesis, indicating the possibility of cross-talk between the TLR/IL-B and Bmp signaling pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ECSIT homolog; Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial; likely ortholog of mouse signaling intermediate in Toll pathway evolutionarily conserved; Protein SITPEC; signaling intermediate in Toll pathway-evolutionarily conserved
Gene Aliases: ECSIT; SITPEC
UniProt ID: (Human) Q9BQ95, (Rat) Q5XIC2, (Mouse) Q9QZH6
Entrez Gene ID: (Human) 51295, (Rat) 300447, (Mouse) 26940
mitochondrial respiratory chain complex I assembly
innate immune response
regulation of oxidoreductase activity
oxidation-reduction process
mesoderm formation
regulation of transcription from RNA polymerase II promoter
transmembrane receptor protein serine/threonine kinase signaling pathway
BMP signaling pathway
immune system process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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