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EDC3 Antibody (PA5-89418) in WB
Western blot analysis of EDC3 in extracts of various cell lines. Samples were incubated with EDC3 Polyclonal antibody (Product # PA5-89418) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-89418
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 159-508 of human EDC3 (NP_0793592)
Conjugate
Form
Liquid
Concentration
0.23 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2805560
Product Specific Information
Immunogen sequence: HNSWSSSSRH PNQATPKKSG LKNGQMKNKD DECFGDDIEE IPDTDFDFEG NLALFDKAAV FEEIDTYERR SGTRSRGIPN ERPTRYRHDE NILESEPIVY RRIIVPHNVS KEFCTDSGLV VPSISYELHK KLLSVAEKHG LTLERRLEMT GVCASQMALT LLGGPNRLNP KNVHQRPTVA LLCGPHVKGA QGISCGRHLA NHDVQVILFL PNFVKMLESI TNELSLFSKT QGQQVSSLKD LPTSPVDLVI NCLDCPENVF LRDQPWYKAA VAWANQNRAP VLSIDPPVHE VEQGIDAKWS LALGLPLPLG EHAGRIYLCD IGIPQQVFQE VGINYHSPFG CKFVIPLHSA; Positive Samples: U-87MG, MCF-7, HeLa, Mouse pancreas; Cellular Location: Cytoplasm, P-body
Target Information
This gene encodes a protein that is important in mRNA degradation. The encoded protein is a component of a decapping complex that promotes efficient removal of the monomethylguanosine (m7G) cap from mRNAs, as part of the 5' to 3' mRNA decay pathway. Mutations in this gene have been identified in human patients with an autosomal recessive form of intellectual disability.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: enhancer of mRNA decapping 3; enhancer of mRNA decapping 3 homolog; Enhancer of mRNA-decapping protein 3; hYjeF_N2; hYjeF_N2-15q23; LSM16 homolog; LSM16 homolog (EDC3, S. cerevisiae); yjeF domain containing; YjeF domain-containing protein 1; YjeF N-terminal domain-containing protein 2; YjeF_N2
Gene Aliases: AA517853; EDC3; LSM16; MRT50; PP844; RGD1306898; YJDC; YJEFN2
UniProt ID: (Human) Q96F86, (Mouse) Q8K2D3
Entrez Gene ID: (Human) 80153, (Rat) 315708, (Mouse) 353190
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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