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EDC4 Antibody (PA5-30485) in ICC/IF
Immunofluorescent analysis of EDC4 (green) in Hela cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and blocked with 2% BSA in PBS for 60 minutes at room temperature. Cells were stained with a EDC4 polyclonal antibody (Product # PA5-30485) at a dilution of 2 µg/mL in staining buffer for 3 hour at room temperature, and then incubated with a Goat anti-rabbit IgG Secondary Antibody, Alexa Fluor 488 conjugate (Product # A32731) at a dilution of 1:1000 for 1 hour a... View More
Product Details
PA5-30485
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 903 and 1181 of Human EDC4
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2547959
Product Specific Information
Recommended positive controls: NT2D1, IMR32, U87-MG, MCF-7, mouse brain.
Predicted reactivity: Mouse (96%), Rat (97%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
In the process of mRNA degradation, seems to play a role in mRNA decapping. Component of a complex containing DCP2 and DCP1A which functions in decapping of ARE-containing mRNAs. Promotes complex formation between DCP1A and DCP2. Enhances the catalytic activity of DCP2 (in vitro).
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Autoantigen Ge-1; Autoantigen RCD-8; Enhancer of mRNA-decapping protein 4; Hedls; Human enhancer of decapping large subunit
Gene Aliases: BC022641; EDC4; Ge-1; GE1; HEDL5; HEDLS; RCD-8; RCD8
UniProt ID: (Human) Q6P2E9, (Mouse) Q3UJB9
Entrez Gene ID: (Human) 23644, (Mouse) 234699
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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