Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 2
EEF1A1 Antibody (PA5-115161) in ICC/IF
Immunocytochemistry analysis of EEF1A1 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115161) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Product Details
PA5-115161
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human EEF1A1(Accession P68104), corresponding to amino acid residues H136-D186.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2899797
Product Specific Information
Antibody detects endogenous levels of total EEF1A1.
Target Information
This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. With PARP1 and TXK, forms a complex that acts as a T helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: cervical cancer suppressor 3; CTCL tumor antigen; eEF1A-1; EF-1 alpha2; EF-1-alpha-1; EF-Tu; EF1a-like protein; EF1A1; elongation factor 1 A-1; elongation factor 1 alpha subunit; Elongation factor 1-alpha 1; Elongation factor Tu; Eukaryotic elongation factor 1 A-1; eukaryotic translation elongation factor 1 alpha 1-like 14; eukaryotic translation elongation factor 1 alpha 2 like 1; glucocorticoid receptor AF-1 specific elongation factor; GRAF 1EF; leukocyte receptor cluster (LRC) member 7; Leukocyte receptor cluster member 7; prostate tumor-inducing protein 1; translation elongation factor 1 alpha 1-like 14
Gene Aliases: CCS-3; CCS3; EE1A1; EEF-1; EEF1A; eEF1A-1; EEF1A1; Eef1a2; Eef1a2l1; EF-Tu; EF1A; GRAF-1EF; HNGC:16303; LENG7; PTI1; SI
UniProt ID: (Human) P68104, (Mouse) P10126, (Rat) P62630
Entrez Gene ID: (Human) 1915, (Mouse) 13627, (Rat) 171361
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support