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EEF1E1 Antibody (MA5-24737) in ICC/IF
Immunocytochemistry analysis of EEF1E1 in Hela (Human Cervical epithelial adenocarcinoma cell line) cells. Samples were incubated with EEF1E1 monoclonal antibody (Product # MA5-24737) using a dilution of 1:25 followed by Dylight® 488-conjugated goat anti-mouse IgG at a dilution of 1:200 (green). Cells were 4% paraformaldehyde-fixed and 0.1% Triton X-100 permeabilized. Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red). The nuclear counter... View More
Product Details
MA5-24737
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG2b, kappa
Class
Monoclonal
Type
Antibody
Clone
1494CT639.147.31
Immunogen
human EEF1E1 recombinant protein
Conjugate
Form
Liquid
Concentration
0.7 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2717212
Target Information
This gene encodes a multifunctional protein that localizes both in the cytoplasm and in the nucleus. In the cytoplasm, the encoded protein is an auxiliary component of the macromolecular aminoacyl-tRNA synthase complex. However, its mouse homolog has been shown to translocate to the nucleus in response to DNA damage and plays a positive role in ATM/ATR-mediated p53 activation. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Aminoacyl tRNA synthetase complex-interacting multifunctional protein 3; ARS-interacting multifunctional protein 3; Elongation factor p18; Eukaryotic translation elongation factor 1 epsilon-1; Multisynthase complex auxiliary component p18; multisynthetase complex auxiliary component p18; p18 component of aminoacyl-tRNA synthetase complex
Gene Aliases: 1110003A02Rik; AIMP3; EEF1E1; P18
UniProt ID: (Human) O43324, (Mouse) Q9D1M4
Entrez Gene ID: (Human) 9521, (Mouse) 66143
translational elongation
tRNA aminoacylation for protein translation
glutathione metabolic process
negative regulation of cell proliferation
positive regulation of apoptotic process
positive regulation of DNA damage response, signal transduction by p53 class mediator
positive regulation of cellular senescence
DNA repair
translation
embryo development
positive regulation of apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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