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Product Details
PA5-120913
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 406-725 of human EEF2K (NP_037434.1).
Conjugate
Form
Liquid
Concentration
0.86 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2914485
Product Specific Information
Positive test controls include: HepG2, HeLa, LOVO, 22Rv1, 293T, Mouse spleen.
Immunogen sequence: KLDHLHWPVF SDLDNMASRD HDHLDNHRES ENSGDSGYPS EKRGELDDPE PREHGHSYSN RKYESDEDSL GSSGRVCVEK WNLLNSSRLH LPRASAVALE VQRLNALDLE KKIGKSILGK VHLAMVRYHE GGRFCEKGEE WDQESAVFHL EHAANLGELE AIVGLGLMYS QLPHHILADV SLKETEENKT KGFDYLLKAA EAGDRQSMIL VARAFDSGQN LSPDRCQDWL EALHWYNTAL EMTDCDEGGE YDGMQDEPRY MMLAREAEML FTGGYGLEKD PQRSGDLYTQ AAEAAMEAMK GRLANQYYQK AEEAWAQMEE
Target Information
EEF2K is a highly conserved Serine/Threoning kinase in the calmodulin signaling pathway whose activity is increased in cancers including breast cancer. EEF2K (eukaryotic elongation factor 2 kinase) is a threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Up activation, by a variety of upstream kinases including AMPK or TRPM7, EEF2K phosphorylates the elongation factor EEF2 at a single site. Then, EEF2K renders EEF2 unable to bind ribosomes and inactive - in turn, the rate of protein synthesis is reduced.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase; calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase; calmodulin-dependent protein kinase III; EC 2.7.11.20; eEF-2 kinase; EF2K; elongation factor-2 kinase; euk; eukaroytic elongation factor 2 kinase; Eukaryotic elongation factor 2 kinase; eukaryotic elongation factor-2 kinase; kinase eEF2K
Gene Aliases: C86191; eEF-2K; EEF2K; HSU93850
UniProt ID: (Human) O00418, (Mouse) O08796
Entrez Gene ID: (Human) 29904, (Mouse) 13631
protein kinase activity
elongation factor-2 kinase activity
calcium ion binding
calmodulin binding
ATP binding
translation factor activity, RNA binding
nucleotide binding
protein serine/threonine kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
response to ischemia
translational elongation
regulation of protein autophosphorylation
cellular response to insulin stimulus
negative regulation of apoptotic process
positive regulation of endocytosis
protein autophosphorylation
positive regulation of synapse assembly
positive regulation of dendritic spine morphogenesis
cellular response to calcium ion
cellular response to cAMP
cellular response to anoxia
cellular response to brain-derived neurotrophic factor stimulus
response to prolactin
protein phosphorylation
phosphorylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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