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A suggested positive control is human spleen tissue lysate.
PA5-20918 can be used with blocking peptide PEP-1032.
EFHD1, also known as Swiprosin-2 or SWS2, is an EF-hand and coiled-coil-containing adaptor protein identified in a subtractive hybridization study using a neuronal cell line established from the cerebellum of an adult p53-null mouse, however further study indicated no difference in normal mice. Its mRNA is widely expressed, with its expression in brain undetectable at embryonic stages, with increasing levels from postnatal to adult development. In situ hybridization showed expression in neurons but not white matter of the cerebellum and cerebrum. EHFD1 is also highly expressed in testes, ovary, and the collecting ducts of the kidney, suggesting that in non-neuronal cells, EFHD1 may be involved in gametogenesis and water-reabsorbtion.
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Protein Aliases: EF hand domain containing 1; EF-hand domain family, member D1; EF-hand domain-containing protein 1; EF-hand domain-containing protein D1; Mitocalcin; Swiprosin-2
Gene Aliases: 4931430I01Rik; AI452351; EFHD1; mitocalcin; MST133; MSTP133; PP3051; RGD1559565; SWS2
UniProt ID: (Human) Q9BUP0, (Mouse) Q9D4J1
Entrez Gene ID: (Human) 80303, (Mouse) 98363, (Rat) 501181
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