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EIF2A Antibody (600-101-ML7) in ICC/IF
Immunofluorescence of Goat Anti-EIF2A Antibody. Cells: HeLa Cells. Fixative: 4% PFA. Permeabilization: 0.3% Triton X-100. Primary Antibody: Anti-EIF2A at 5µg/mL overnight at 2-8°C. Secondary Antibody: Donkey Anti-Goat IgG DyLight™488 (p/n 605-741-125) at 5µg/mL for 1hr at RT. Nuclear Counterstain: DAPI. Staining: (A) DAPI. (B) Anti-EIF2A and DyLight™488. (C) merge of A+B. (D). Secondary only. Localization: cytosol, extracellular >> mitochondria.
Product Details
600-101-ML7
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-EIF2A antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to a near N-terminal portion of human EIF2A conjugated to Keyhole Limpet Hemocyanin (KLH).
Conjugate
Form
Liquid
Concentration
1.08 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against human EIF2A. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 86.7% homology with the immunizing sequence to mouse.
Target Information
EIF2A is a 65-kD protein that catalyzes the formation of puromycin-sensitive 80S preinitiation complexes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 65 kDa eukaryotic translation initiation factor 2A; eIF-2A; Eukaryotic translation initiation factor 2A; eukaryotic translation initiation factor 2A, 65kDa
Gene Aliases: CDA02; D030048D22; D3Ertd194e; EIF-2A; EIF2A; MST089; MSTP004; MSTP089
UniProt ID: (Human) Q9BY44, (Mouse) Q8BJW6
Entrez Gene ID: (Human) 83939, (Mouse) 229317
Performance Guarantee
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