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ELK1 Antibody (PA5-87504) in WB
Western blot analysis of ELK1 in extracts of various cell lines. Samples were incubated with ELK1 Polyclonal antibody (Product # PA5-87504) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
PA5-87504
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 201-297 of human ELK1 (NP_0052202)
Conjugate
Form
Liquid
Concentration
1.53 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2804207
Product Specific Information
Immunogen sequence: PSPLEACLEA EEAGLPLQVI LTPPEAPNLK SEELNVEPGL GRALPPEVKV EGPKEELEVA GERGFVPETT KAEPEVPPQE GVPARLPAVV MDTAGQA
Positive Samples: HeLa, Jurkat, HT-29, K-562, Mouse liver, Rat brain; Cellular Location: Nucleus
Target Information
ELK1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors. ELK1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its C-terminus. Phosphorylation at these sites, particularly Ser383, is critical for transcriptional activation by ELK1. ELK1 appears to be a direct target of activated MAP kinase. Biochemical studies indicate that ELK1 is a good substrate for MAP kinase, the kinetics of ELK1 phosphorylation and activation correlate with MAP kinase activity, and interfering mutants of MAP kinase block ELK1 activation in vivo. ELK1 is a nuclear target for the ras-raf-MAPK signaling cascade. Alternatively spliced transcript variants encoding the same protein have been found for ELK1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ELK 1; ELK1, member of ETS oncogene family; ETS domain-containing protein Elk-1; ETS-like gene 1; Oncogene Elk1; tyrosine kinase (ELK1) oncogene
Gene Aliases: Elk-1; ELK1
UniProt ID: (Human) P19419, (Mouse) P41969, (Rat) A4GTP4
Entrez Gene ID: (Human) 2002, (Mouse) 13712, (Rat) 314436
RNA polymerase II core promoter proximal region sequence-specific DNA binding
core promoter binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II transcription factor binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
DNA binding
double-stranded DNA binding
sequence-specific DNA binding
winged helix/forkhead transcription factor
transcription from RNA polymerase II promoter
response to light stimulus
cell differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
cellular response to testosterone stimulus
cellular response to gamma radiation
response to fibroblast growth factor
positive regulation of neuron death
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
cellular response to lipid
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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