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Invitrogen
ENO1 Recombinant Rabbit Monoclonal Antibody (063)
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 4
ENO1 Antibody (MA5-30367) in ICC/IF
Immunofluorescence analysis of ENO1 was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with ENO1 Polyclonal Antibody (Product # MA5-30367) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Pr... View More
Product Details
MA5-30367
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
063
Immunogen
Recombinant Human ENO1/Enolase 1/alpha-enolase protein (Met1-Lys434)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2786110
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This antibody has specificity for Human ENO1/Enolase 1/alpha-enolase.
Target Information
Enolase (2-phosphogly-cerate hydrolyase or phosphopyruvate hydrates) is a glycolytic enzyme that catalyzes the dehydration and conversion of 2-phosphoglycerate to phosphoenolpyruvate. It comprises three distinct subunits, alpha, beta and gamma. Non Neuronal Enolase(ENO1) is an isoform of mammalian enolase and is composed of 2 alpha subunits. The gene for ENO1 also encodes a shorter monomeric structural lens protein, tau-crystallin. Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.Used as a diagnostic marker for many tumors and, in the heterodimeric form, alpha/gamma, as a marker for hypoxic brain injury after cardiac arrest. Also marker for endometriosis. Antibodies against alpha-enolase are present in sera from patients with cancer-associated retinopathy syndrome (CAR), a progressive blinding disease which occurs in the presence of systemic tumor growth, primarily small-cell carcinoma of the lung and other malignancies. Is identified as an autoantigen in Hashimoto encephalopathy (HE) a rare autoimmune disease associated with Hashimoto thyroiditis (HT). HT is a disorder in which destructive processes overcome the potential capacity of thyroid replacement leading to hypothyroidism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 2-phospho-D-glycerate hydro-lyase; alpha enolase like 1; Alpha-enolase; C-myc promoter-binding protein; enoa; Enolase 1; enolase 1, (alpha); enolase-alpha; epididymis secretory protein Li 17; MBP-1; MPB-1; MYC promoter-binding protein 1; NNE; Non-neural enolase; Phosphopyruvate hydratase; Plasminogen-binding protein; tau-crystallin
Gene Aliases: ENO1; ENO1L1; HEL-S-17; MBPB1; MPB1; NNE; PPH
UniProt ID: (Human) P06733
Entrez Gene ID: (Human) 2023
magnesium ion binding
DNA binding
transcription factor activity, sequence-specific DNA binding
transcription corepressor activity
phosphopyruvate hydratase activity
protein binding
poly(A) RNA binding
protein heterodimerization activity
GTPase binding
cadherin binding involved in cell-cell adhesion
lyase
negative regulation of transcription from RNA polymerase II promoter
in utero embryonic development
gluconeogenesis
transcription, DNA-templated
response to virus
negative regulation of cell growth
negative regulation of transcription, DNA-templated
positive regulation of binding
canonical glycolysis
cell-cell adhesion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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