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ENOPH1 Antibody (CF810868) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ENOPH1 (RC209510, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-ENOPH. Positive lysates LY4120°C0 (100 µg) and LC4120°C0 (20 µg) can be purchased separately from OriGene.
Product Details
CF810868
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
OTI4E1
Immunogen
Full length human recombinant protein of human ENOPH1 produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 2,3-diketo-5-methylthio-1-phosphopentane phosphatase; 2,3-diketo-5-methylthio-1-phosphopentane phosphatase {ECO:0000255|HAMAP-Rule:MF_03117}; acireductone synthase; E-1 enzyme; Enolase-phosphatase E1; enolase-phosphatase E1 {ECO:0000255|HAMAP-Rule:MF_03117}; ENOPH1; MASA homolog; MASA homolog {ECO:0000255|HAMAP-Rule:MF_03117}
Gene Aliases: 2310057D15Rik; BB183658; C81437; E1; ENOPH1; MASA; MST145; MSTP145; mtnC; RGD1309016
UniProt ID: (Human) Q9UHY7, (Mouse) Q8BGB7, (Rat) Q5PPH0
Entrez Gene ID: (Human) 58478, (Mouse) 67870, (Rat) 305177
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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