Proteintech

ERK1/2 Polyclonal Antibody

ERK1/2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 18

ERK1/2 Antibody (51068-1-AP) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ERK1/2 antibody (Product # 51068-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

ERK1/2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ERK1/2 Antibody in Flow Cytometry (Flow)

Product Image Gallery

Product Details

51068-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:6,000

View 19 publications 19 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

Immunocytochemistry (ICC/IF)

1:10-1:100

Flow Cytometry (Flow)

Assay-dependent

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.37 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-43 kDa.

Target Information

ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Promotion of the genipin crosslinked chitosan-fiber hydrogel loaded with sustained release of clemastine fumarate in diabetic wound repair.

International journal of biological macromolecules

Zuo R,Shi J,Jiang S,Chu M,Wang Q,Kong L,Kang Q,Guo Y,Guan J

Tue Jan 31 00:00:00 EST 2023

Silica nanoparticles induce pulmonary damage in rats via VEGFC/D-VEGFR3 signaling-mediated lymphangiogenesis and remodeling.

Toxicology

Yu Y,Pan Y,Chang B,Zhao X,Qu K,Song Y

Sat Jul 01 00:00:00 EDT 2023

Biochemical and clinical effects of RPS20 expression in renal clear cell carcinoma.

Oncology reports

Shen C,Chen Z,Zhang Y,Xu W,Peng R,Jiang J,Zuo W,Fan Y,Zheng B

Sun Jan 01 00:00:00 EST 2023

Anoikis patterns exhibit distinct prognostic and immune landscapes in Osteosarcoma.

International immunopharmacology

Zhang Z,Zhu Z,Fu J,Liu X,Mi Z,Tao H,Fan H

Wed Feb 01 00:00:00 EST 2023

Anoikis patterns exhibit distinct prognostic and immune landscapes in Osteosarcoma.

International immunopharmacology

Zhang Z,Zhu Z,Fu J,Liu X,Mi Z,Tao H,Fan H

Wed Feb 01 00:00:00 EST 2023

Low Pi stress enhances the sensitivity of hepatocellular carcinoma to sorafenib.

Biochemical pharmacology

Bi QC,Deng ZQ,Lv YF,Liu Y,Xie CS,He YQ,Tang Q

Sat Jul 01 00:00:00 EDT 2023

Effects of Poria cocos extract on metabolic dysfunction-associated fatty liver disease via the FXR/PPARα-SREBPs pathway.

Frontiers in pharmacology

He J,Yang Y,Zhang F,Li Y,Li X,Pu X,He X,Zhang M,Yang X,Yu Q,Qi Y,Li X,Yu J

Mon Jun 05 00:00:00 EDT 2023

Hyaluronic Acid-Modified Nanoparticles Self-Assembled from Linoleic Acid-Conjugated Chitosan for the Codelivery of miR34a and Doxorubicin in Resistant Breast Cancer.

Molecular pharmaceutics

Yang X,Shang P,Ji J,Malichewe C,Yao Z,Liao J,Du D,Sun C,Wang L,Tang YJ,Guo X

Mon Jan 03 00:00:00 EST 2022

Tetrahydrocurcumin improves lipopolysaccharide-induced myocardial dysfunction by inhibiting oxidative stress and inflammation via JNK/ERK signaling pathway regulation.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Zhu H,Zhang L,Jia H,Xu L,Cao Y,Zhai M,Li K,Xia L,Jiang L,Li X,Zhou Y,Liu J,Yu S,Duan W

Thu Sep 01 00:00:00 EDT 2022

Suppression of AGTR1 Induces Cellular Senescence in Hepatocellular Carcinoma Through Inactivating ERK Signaling.

Frontiers in bioengineering and biotechnology

Wang H,Cui Y,Gong H,Xu J,Huang S,Tang A

Tue Aug 02 00:00:00 EDT 2022

Tangeretin prevents cognitive deficit in delirium through activating RORα/γ-E4BP4 axis in mice.

Biochemical pharmacology

Chen M,Xiao Y,Zhang F,Du J,Zhang L,Li Y,Lu D,Wang Z,Wu B

Tue Nov 01 00:00:00 EDT 2022

ETV6 Regulates Hemin-Induced Erythroid Differentiation of K562 Cells through Mediating the Raf/MEK/ERK Pathway.

Biological & pharmaceutical bulletin

Li Z,Sun MZ,Lv X,Guo C,Liu S

Mon Apr 04 00:00:00 EDT 2022

Effect of propiconazole on neutrophil extracellular traps formation: Assessing the role of autophagy.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

Ma Y,Li S,Ye S,Hu D,Luo S,Wei L,Xiao F

Sat Oct 01 00:00:00 EDT 2022

Spiropachysine A suppresses hepatocellular carcinoma proliferation by inducing methuosis in vitro and in vivo.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Fang Y,Zhong T,Yang L,Luo F,Li Q,Wang D,Li Q,Fan Y,Yang X

Wed Jul 20 00:00:00 EDT 2022

Leptin brain entry via a tanycytic LepR-EGFR shuttle controls lipid metabolism and pancreas function.

Nature metabolism

Duquenne M,Folgueira C,Bourouh C,Millet M,Silva A,Clasadonte J,Imbernon M,Fernandois D,Martinez-Corral I,Kusumakshi S,Caron E,Rasika S,Deliglia E,Jouy N,Oishi A,Mazzone M,Trinquet E,Tavernier J,Kim YB,Ory S,Jockers R,Schwaninger M,Boehm U,Nogueiras R,Annicotte JS,Gasman S,Dam J,Prévot V

Sun Aug 01 00:00:00 EDT 2021

Guifu Dihuang Pills Ameliorated Mucus Hypersecretion by Suppressing Muc5ac Expression and Inactivating the ERK-SP1 Pathway in Lipopolysaccharide/Cigarette Smoke-Induced Mice.

Evidence-based complementary and alternative medicine : eCAM

Zhang H,Yu W,Ji L,Zhong Y,Lin Y,Ying H,Yu C,Li C

Wed Nov 17 00:00:00 EST 2021

Rubioncolin C, a natural naphthohydroquinone dimer isolated from Rubia yunnanensis, inhibits the proliferation and metastasis by inducing ROS-mediated apoptotic and autophagic cell death in triple-negative breast cancer cells.

Journal of ethnopharmacology

Li L,Wang J,Feng L,Fan J,Wang J,Tan N,Wang Z

Wed Sep 15 00:00:00 EDT 2021

The PI3Kα inhibitor DFX24 suppresses tumor growth and metastasis in non-small cell lung cancer via ERK inhibition and EPHB6 reactivation.

Pharmacological research

Fan YH,Ding HW,Kim D,Liu JY,Hong JY,Xu YN,Wang D,Yang XS,Lee SK

Thu Oct 01 00:00:00 EDT 2020

Suhuang Antitussive Capsules-Ameliorative Effects on LPS-Induced Sputum Obstruction in Mice Through Promoting HGF Secretion.

Frontiers in pharmacology

Tong X,Liang R,Jia Y,Qin W,Guo C,Wu X,Wang Z,Chen D,Tan N

Wed Sep 30 00:00:00 EDT 2020

Siglec-F is induced by granulocyte-macrophage colony-stimulating factor and enhances interleukin-4-induced expression of arginase-1 in mouse macrophages.

Immunology

Tateyama H,Murase Y,Higuchi H,Inasaka Y,Kaneoka H,Iijima S,Nishijima KI

Sun Dec 01 00:00:00 EST 2019

Formononetin inhibits colon carcinoma cell growth and invasion by microRNA‑149‑mediated EphB3 downregulation and inhibition of PI3K/AKT and STAT3 signaling pathways.

Molecular medicine reports

Wang AL,Li Y,Zhao Q,Fan LQ

Fri Jun 01 00:00:00 EDT 2018

Formononetin inhibits colon carcinoma cell growth and invasion by microRNA‑149‑mediated EphB3 downregulation and inhibition of PI3K/AKT and STAT3 signaling pathways.

Molecular medicine reports

Wang AL,Li Y,Zhao Q,Fan LQ

Fri Jun 01 00:00:00 EDT 2018

Urokinase-type plasminogen activator receptor signaling is critical in nasopharyngeal carcinoma cell growth and metastasis.

Cell cycle (Georgetown, Tex.)

Bao YN,Cao X,Luo DH,Sun R,Peng LX,Wang L,Yan YP,Zheng LS,Xie P,Cao Y,Liang YY,Zheng FJ,Huang BJ,Xiang YQ,Lv X,Chen QY,Chen MY,Huang PY,Guo L,Mai HQ,Guo X,Zeng YX,Qian CN

Tue Jan 20 00:00:00 EST 2015

Urokinase-type plasminogen activator receptor signaling is critical in nasopharyngeal carcinoma cell growth and metastasis.

Cell cycle (Georgetown, Tex.)

Bao YN,Cao X,Luo DH,Sun R,Peng LX,Wang L,Yan YP,Zheng LS,Xie P,Cao Y,Liang YY,Zheng FJ,Huang BJ,Xiang YQ,Lv X,Chen QY,Chen MY,Huang PY,Guo L,Mai HQ,Guo X,Zeng YX,Qian CN

Tue Jan 20 00:00:00 EST 2015

A systems biology-based classifier for hepatocellular carcinoma diagnosis.

PloS one

Zhang Y,Wang S,Li D,Zhnag J,Gu D,Zhu Y,He F

Tue Dec 06 00:00:00 EST 2011

A systems biology-based classifier for hepatocellular carcinoma diagnosis.

PloS one

Zhang Y,Wang S,Li D,Zhnag J,Gu D,Zhu Y,He F

Tue Dec 06 00:00:00 EST 2011

Bioinformatics

Protein Aliases: ERK-1; ERK-2; erk1 erk2; ERK1b; ERT1; ERT2; Extracellular signal-regulated kinase 1; Extracellular signal-regulated kinase 2; extracellular signal-related kinase 1; extracellular-signal-regulated kinase 2; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 2; MAP kinase 3; MAP kinase isoform p42; MAP kinase isoform p44; MAPK 1; MAPK 2; MAPK 3; Microtubule-associated protein 2 kinase; mitogen activated protein kinase 1; mitogen-activated 3; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 2; Mitogen-activated protein kinase 3; MNK1; p42 MAP Kinase; p42-MAPK; p44 MAP kinase; p44-ERK1; p44-MAPK; pp42/MAP kinase; protein tyrosine kinase ERK2

View more View less

Gene Aliases: 9030612K14Rik; AA407128; AU018647; C78273; ERK; ERK-1; ERK-2; ERK1; ERK2; ERT1; ERT2; Esrk1; HS44KDAP; HUMKER1A; Mapk; MAPK1; MAPK2; MAPK3; MNK1; Mtap2k; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; p44; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM1; PRKM2; PRKM3

View more View less

UniProt ID: (Human) P28482, (Human) P27361, (Mouse) P63085, (Rat) P63086, (Rat) P21708, (Mouse) Q63844

View more View less

Entrez Gene ID: (Human) 5594, (Human) 5595, (Mouse) 26413, (Rat) 116590, (Rat) 50689, (Mouse) 26417

View more View less

Function(s)

phosphotyrosine binding DNA binding protein serine/threonine kinase activity MAP kinase activity protein binding ATP binding transcription factor binding RNA polymerase II carboxy-terminal domain kinase activity kinase activity phosphatase binding mitogen-activated protein kinase kinase kinase binding identical protein binding scaffold protein binding nucleotide binding protein kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein kinase binding non-receptor serine/threonine protein kinase

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

ERK1/2 Polyclonal Antibody

ERK1/2 Antibody (51068-1-AP) in ICC/IF

Product Details

51068-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!