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Endothelin 3 Antibody (PA5-119887) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of of EDN3 in MG-63 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Endothelin 3 Polyclonal Antibody (Product # PA5-119887) at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Ale... View More
Product Details
PA5-119887
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide within human Endothelin 3 aa 80-130
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
TBS, pH 7.4, with 50% glycerol, 0.2% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2913459
Product Specific Information
Positive Control: Raji cell lysate, 293 cell lysate, MCF-7 cell lysate, SW480 cell lysate, MG-63, rat placenta tissue, human placenta tissue, mouse placenta tissue.
Subcellular Location: Secreted.
Target Information
The protein encoded by this gene is a member of the endothelin family. Endothelins are endothelium-derived vasoactive peptides involved in a variety of biological functions. The active form of this protein is a 21 amino acid peptide processed from the precursor protein. The active peptide is a ligand for endothelin receptor type B (EDNRB). The interaction of this endothelin with EDNRB is essential for development of neural crest-derived cell lineages, such as melanocytes and enteric neurons. Mutations in this gene and EDNRB have been associated with Hirschsprung disease (HSCR) and Waardenburg syndrome (WS), which are congenital disorders involving neural crest-derived cells. Four alternatively spliced transcript variants encoding three distinct isoforms have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Endothelin-3; ET 3; ET-3; lethal spotting; MGC15067; MGC61498; PPET3; Preproendothelin 3; Preproendothelin-3; RP4-614C15.1
Gene Aliases: 114CH19; EDN3; ET-3; ET3; HSCR4; ls; PPET3; preproET-3; RGD1564825; tmgc48; WS4B
UniProt ID: (Human) P14138, (Mouse) P48299, (Rat) P13207
Entrez Gene ID: (Human) 1908, (Mouse) 13616, (Rat) 366270
neural crest cell migration
positive regulation of leukocyte chemotaxis
regulation of systemic arterial blood pressure by endothelin
cellular calcium ion homeostasis
signal transduction
cell surface receptor signaling pathway
cell-cell signaling
multicellular organism development
blood circulation
positive regulation of cell proliferation
positive regulation of heart rate
regulation of gene expression
cellular magnesium ion homeostasis
artery smooth muscle contraction
vein smooth muscle contraction
regulation of vasoconstriction
peptide hormone secretion
neuron differentiation
melanocyte differentiation
neutrophil chemotaxis
vasoconstriction
positive regulation of MAP kinase activity
positive regulation of cell differentiation
positive regulation of mitotic nuclear division
positive regulation of hormone secretion
inositol phosphate-mediated signaling
regulation of developmental pigmentation
positive regulation of potassium ion transmembrane transport
regulation of cell migration
negative regulation of hormone secretion
regulation of neurotransmitter secretion
regulation of blood vessel size
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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