Invitrogen

EpCAM Monoclonal Antibody (323/A3)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

7 References

View all (275) EpCAM (CD326) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (5)
Advanced Verification (1)

EpCAM Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 6

EpCAM Antibody (MA1-10196) in ICC/IF

Immunofluorescence analysis of EpCAM was performed using HT-29 and HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with EpCAM Mouse Monoclonal Antibody (Product # MA1-10196) at 1 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32723) at a dilution of 1:2000... View More

EpCAM Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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EpCAM Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of EpCAM was observed in HT-29 cells in plasma membrane, which is a positive model for EpCAM expression, in comparison to HeLa (null for EpCAM expression) using Anti-EpCAM Mouse Monoclonal Antibody (Product # MA1-10196) in immunofluorescence. {RE}

Product Details

MA1-10196

Applications

Tested Dilution

Publications

Western Blot (WB)

1-4 µg/mL

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1-10 µg/mL

Immunocytochemistry (ICC/IF)

1-10 µg/mL

View 2 publications 2 publications

Flow Cytometry (Flow)

1-4 µg/mL

View 2 publications 2 publications

Immunoprecipitation (IP)

1-4 µg/mL

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

323/A3

Immunogen

Human breast cancer MCF-7 cells

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11154429

Product Specific Information

This antibody recognizes an extracellular epitope of CD326 / EpCAM, a marker of epithelial lineages, that is over-expressed in many carcinomas.

This antibody will not cross-react with rat.

Western Blot: non-reducing conditions

Target Information

Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein expressed in the epithelium with a molecular weight of approximately 40 kDa, which functions as an epithelial cell adhesion molecule. Ep-CAM functions as a homotypic calcium-independent cell adhesion molecule, and has a direct impact on cell cycle, proliferation and metabolism of epithelial cells and fibroblasts due to its ability to rapidly induce the proto-oncogene c-myc and the cell cycle regulating genes cyclin A and E. Ep-CAM mediates Ca2+-independent homotypic interactions. Formation of Ep-CAM-mediated adhesions have a negative regulatory effect on adhesions mediated by classic cadherins, which may have strong effects on the differentiation and growth of epithelial cells. Ep-CAM overexpression was suggested to be associated with enhanced epithelial proliferation. Ep-CAM is highly expressed in human carcinomas, and is a marker for tumors of epithelial lineage. Ep-CAM is expressed on baso-lateral cell surface in most simple epithelia and many carcinoma types. Also, Ep-CAM reportedly distinguishes adenocarcinomas from pleural mesotheliomas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Co-existence of epithelioid and fibroblastoid subsets in a sarcomatoid renal carcinoma cell line revealed by clonal studies.

Anticancer research

Hsieh CH,Chen HC,Chang YH,Pang ST,Kuo ML,Chuang CK,Liao SK

MA1-10196 was used in flow cytometry to study the presence in a sarcomatoid renal carcinoma cell line of both epithelioid and fibroblastoid cells

Fri Nov 01 00:00:00 EDT 2013

Enrichment of circulating tumor cells from a large blood volume using leukapheresis and elutriation: proof of concept.

Cytometry. Part B, Clinical cytometry

Eifler RL,Lind J,Falkenhagen D,Weber V,Fischer MB,Zeillinger R

MA1-10196 was used in immunocytochemistry to develop a process involving leukapheresis, elutriation and FACS to isolate circulating tumor cells from large volumes

Tue Mar 01 00:00:00 EST 2011

Overexpression of epithelial cell adhesion molecule in primary, metastatic, and recurrent/chemotherapy-resistant epithelial ovarian cancer: implications for epithelial cell adhesion molecule-specific immunotherapy.

International journal of gynecological cancer : official journal of the International Gynecological Cancer Society

Bellone S,Siegel ER,Cocco E,Cargnelutti M,Silasi DA,Azodi M,Schwartz PE,Rutherford TJ,Pecorelli S,Santin AD

MA1-10196 was used in immunohistochemistry to study the therapeutic implications of epithelial cell adhesion molecule overexpression in epithelial ovarian carcinomas

Wed Jul 01 00:00:00 EDT 2009

EpCAM-specific vaccine response by modified antigen and chimeric costimulatory molecule in cynomolgus monkeys.

Journal of immunotherapy (Hagerstown, Md. : 1997)

Neighbors M,Apt D,Chang JC,Brinkman A,Sipos-Solman I,Ong R,Leong S,Punnonen J

MA1-10196 was used in western blot to examine a novel costimulatory adjuvant for tumor immuno therapy

Mon Sep 01 00:00:00 EDT 2008

Gradients in the liver's extracellular matrix chemistry from periportal to pericentral zones: influence on human hepatic progenitors.

Tissue engineering. Part A

McClelland R,Wauthier E,Uronis J,Reid L

MA1-10196 was used in immunohistochemistry to identify key matrix components required for ex vivo maintenance of human hepatic progenitors

Tue Jan 01 00:00:00 EST 2008

Inhibition of proliferation by PERK regulates mammary acinar morphogenesis and tumor formation.

PloS one

Sequeira SJ,Ranganathan AC,Adam AP,Iglesias BV,Farias EF,Aguirre-Ghiso JA

MA1-10196 was used in immunocytochemistry to study the role of adhesion-dependent regulation of PERK activity in modulating mammary acinar morphogenesis and breast tumorigenesis via inhibition of proliferation

Wed Jul 18 00:00:00 EDT 2007

Genetic vaccines against Ep-CAM break tolerance to self in a limited subset of subjects: initial identification of predictive biomarkers.

European journal of immunology

Elia L,Mennuni C,Storto M,Podda S,Calvaruso F,Salucci V,Aurisicchio L,Scarito A,Ciliberto G,La Monica N,Palombo F

MA1-10196 was used in flow cytometry to evaluate the Ep-CAM genetic vaccination strategy in animal models

Mon May 01 00:00:00 EDT 2006

Bioinformatics

Protein Aliases: Adenocarcinoma-associated antigen; CD326; Cell surface glycoprotein Trop-1; EGP; EGP314; Ep-CAM; Epithelial cell adhesion molecule; Epithelial cell surface antigen; Epithelial glycoprotein; Epithelial glycoprotein 314; hEGP314; human epithelial glycoprotein-2; KS 1/4 antigen; KSA; Major gastrointestinal tumor-associated protein GA733-2; membrane component, chromosome 4, surface marker (35kD glycoprotein); Tumor-associated calcium signal transducer 1

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Gene Aliases: DIAR5; EGP-2; EGP314; EGP40; EPCAM; ESA; GA733-2; HNPCC8; KS1/4; KSA; M1S2; M4S1; MIC18; MK-1; TACSTD1; TROP1

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UniProt ID: (Human) P16422

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Entrez Gene ID: (Human) 4072

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Function(s)

protein binding protein complex binding cadherin binding involved in cell-cell adhesion

Process(es)

ureteric bud development positive regulation of cell proliferation signal transduction involved in regulation of gene expression negative regulation of apoptotic process positive regulation of transcription from RNA polymerase II promoter stem cell differentiation cell-cell adhesion negative regulation of cell-cell adhesion mediated by cadherin positive regulation of cell motility positive regulation of stem cell proliferation

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EpCAM Monoclonal Antibody (323/A3)

EpCAM Antibody (MA1-10196) in ICC/IF

Product Details

MA1-10196

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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