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FGF4 Antibody (PA5-90007) in WB
Western blot analysis of FGF4 in extracts of Rat liver. Samples were incubated with FGF4 Polyclonal antibody (Product # PA5-90007) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Product Details
PA5-90007
Product Specifications
Species Reactivity
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 31-206 of human FGF4 (NP_0019981)
Conjugate
Form
Liquid
Concentration
1.32 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2805887
Product Specific Information
Immunogen sequence: APTAPNGTLE AELERRWESL VALSLARLPV AAQPKEAAVQ SGAGDYLLGI KRLRRLYCNV GIGFHLQALP DGRIGGAHAD TRDSLLELSP VERGVVSIFG VASRFFVAMS SKGKLYGSPF FTDECTFKEI LLPNNYNAYE SYKYPGMFIA LSKNGKTKKG NRVSPTMKVT HFLPRL; Positive Samples: Rat liver
Target Information
Fibroblast growth factor 4 (FGF4) is a member of the fibroblast growth factor (FGF) family that possess broad mitogenic and cell survival activities and play key roles in growth and survival of stem cells during embryogenesis, tissue regeneration, and carcinogenesis. FGF4 was identified by its strong oncogenic transforming activity and is a potent angiogenic factor, expressed in several highly vascularized tumors and also in adult mouse testis, intestine, and brain. Studies on the mouse homolog suggests a function in bone morphogenesis and limb development through the sonic hedgehog (SHH) signaling pathway. Furthermore, FGF4 regulates neural progenitor cell proliferation and neuronal differentiation. Recent studies show a growth-promoting role for FGF4 in human embryonic stem cells and a putative feedback inhibition mechanism by a novel FGF4 splice isoform that may serve to promote differentiation at a later stages of development.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FGF; Fibroblast growth factor; fibroblast growth factor 4; heparin-binding growth factor 1
Gene Aliases: Hst; Hst1
Entrez Gene ID: (Rat) 116499
cartilage condensation
positive regulation of protein phosphorylation
positive regulation of cell proliferation
fibroblast growth factor receptor signaling pathway
mesenchymal cell proliferation
regulation of gene expression
positive regulation of gene expression
stem cell population maintenance
embryonic limb morphogenesis
embryonic hindlimb morphogenesis
odontogenesis of dentin-containing tooth
negative regulation of apoptotic process
positive regulation of transcription from RNA polymerase II promoter
cranial suture morphogenesis
apoptotic process involved in morphogenesis
chondroblast differentiation
positive regulation of ERK1 and ERK2 cascade
regulation of endothelial cell chemotaxis to fibroblast growth factor
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