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FIGURE: 1 / 1
GABPA Antibody (PA5-88319) in WB
Western blot analysis of GABPA in extracts of various cell lines. Samples were incubated with GABPA Polyclonal antibody (Product # PA5-88319) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
PA5-88319
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human GABPA (NP_0020312)
Conjugate
Form
Liquid
Concentration
0.15 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2804826
Product Specific Information
Positive Samples: HeLa, Raji, HepG2, SKOV3, Rat liver; Cellular Location: Nucleus
Immunogen sequence: NLKKLLEPRL QCSLDAHEIC LQDIQLDPER SLFDQGVKTD GTVQLSVQVI SYQGIEPKLN ILEIVKPADT VEVVIDPDAH HAESEAHLVE EAQVITLDGT K
Target Information
This gene encodes one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit. Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, this gene may play a role in the Down Syndrome phenotype. Two transcript variants encoding the same protein have been found for this gene.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GA binding protein transcription factor alpha subunit 60kDa; GA binding protein transcription factor, alpha subunit 60kDa; GA repeat binding protein, alpha; GA-binding protein alpha chain; GABP subunit alpha; human nuclear respiratory factor-2 subunit alpha; nuclear respiratory factor 2 alpha; nuclear respiratory factor 2 alpha subunit; Nuclear respiratory factor 2 subunit alpha; Transcription factor E4TF1-60
Gene Aliases: E4TF1-60; E4TF1A; GABPA; NFT2; Nrf-2alpha; NRF2; NRF2A; RCH04A07
UniProt ID: (Human) Q06546
Entrez Gene ID: (Human) 2551, (Rat) 363735
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
transcription coactivator activity
protein binding
transcription regulatory region DNA binding
protein heterodimerization activity
RNA polymerase II transcription factor activity, sequence-specific DNA binding
winged helix/forkhead transcription factor
negative regulation of transcription from RNA polymerase II promoter
in utero embryonic development
transcription from RNA polymerase II promoter
mitochondrion organization
cell differentiation
negative regulation of megakaryocyte differentiation
positive regulation of transcription from RNA polymerase II promoter
regulation of transcription from RNA polymerase II promoter
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