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GABRA2 Antibody (MA5-27702) in ICC/IF
Immunofluorescent analysis of GABRA2 in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with GABRA2 monoclonal antibody (Product # MA5-27702) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) GABA-A Receptor Alpha2 Antibody, D) Merged i... View More
Product Details
MA5-27702
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
N399/19
Immunogen
Fusion protein amino acids 350-385 (Cytoplasmic C-terminus) of rat GABA-A Receptor Alpha2
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2735191
Product Specific Information
A 1:100 dilution of MA5-27702 was sufficient for detection of GABA-A R, Alpha2 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |Detects approximately 55kDa. Does not cross-react with GABA-A Receptor Alpha1.
This antibody was formerly sold as clone S399-19.
Target Information
GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. Alternatively spliced transcript variants encoding the same protein have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: FLJ97076; GABA(A) receptor subunit alpha-2; GABA(A) receptor, alpha 2; GABAAR subunit alpha-2; GABAARalpha2; gamma-aminobutyric acid; gamma-aminobutyric acid (GABA) A receptor, alpha 2; gamma-aminobutyric acid (GABA-A) receptor, subunit alpha 2; gamma-aminobutyric acid A receptor alpha 2; gamma-aminobutyric acid receptor A2 subunit; Gamma-aminobutyric acid receptor subunit alpha-2
Gene Aliases: C630048P16Rik; Gabra-2; GABRA2
UniProt ID: (Human) P47869, (Mouse) P26048
Entrez Gene ID: (Human) 2555, (Mouse) 14395, (Rat) 289606
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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