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GABRA5 Antibody (MA5-27700) in ICC/IF
Immunofluorescent analysis of GABRA5 in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with GABRA5 monoclonal antibody (Product # MA5-27700) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) GABA-A Receptor Alpha5 Antibody, D) Merged i... View More
Product Details
MA5-27700
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
N415/24
Immunogen
Fusion protein amino acids 368-419 (last cytoplasmic domain before extracellular C-terminus) of human GABA A Receptor Alpha5.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2735193
Product Specific Information
A 1:100 dilution of MA5-27700 was sufficient for detection of GABA-A R, Alpha5 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |Detects approximately 55kDa. Does not cross-react with GABA A Receptor Alpha2 or other GABA-A Receptors based on KO validation data.
This antibody was formerly sold as clone S415-24.
Target Information
GABRA5 is a part of GABA, a major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. Transcript variants utilizing three different alternative non-coding first exons have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GABA(A) receptor subunit alpha-5; GABA-A receptor alpha-5 subunit; GABAA receptor alpha5 subunit; GABAAR subunit alpha-5; gamma-aminobutyric acid; gamma-aminobutyric acid A receptor, alpha 5; gamma-aminobutyric acid (GABA) A receptor, alpha 5; gamma-aminobutyric acid (GABA-A) receptor, subunit alpha 5; gamma-aminobutyric acid A receptor, alpha 5; Gamma-aminobutyric acid receptor subunit alpha-5; ligand-gated ion channel subunit
Gene Aliases: A230018I05Rik; Gabra-5; GABRA5
UniProt ID: (Human) P31644, (Rat) P19969, (Mouse) Q8BHJ7
Entrez Gene ID: (Human) 2558, (Rat) 29707, (Mouse) 110886
behavioral fear response
positive regulation of defense response to virus by host
transport
signal transduction
gamma-aminobutyric acid signaling pathway
chemical synaptic transmission
brain development
sensory perception of sound
associative learning
ion transmembrane transport
negative regulation of neuron apoptotic process
inner ear receptor cell development
innervation
cochlea development
mitophagy in response to mitochondrial depolarization
xenophagy
chloride transmembrane transport
regulation of neuron apoptotic process
neuron development
ion transport
chloride transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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