Invitrogen

GAPDH Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

4 Published Figures

15 References

View all (122) GAPDH antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

GAPDH Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 17

GAPDH Antibody (PA1-16777) in ICC/IF

Immunofluorescence analysis of GAPDH was performed using 70% confluent log phase NIH/3T3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with GAPDH Rabbit Polyclonal Antibody (Product # PA1-16777) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product ... View More

GAPDH Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

GAPDH Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with alpha GAPDH siRNA and loss of signal was observed in Western Blot using Anti-GAPDH Polyclonal Antibody (Product # PA1-16777). {KD}

Product Details

PA1-16777

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:10,000

View 13 publications 13 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500

Immunocytochemistry (ICC/IF)

1:50-1:200

View 2 publications 2 publications

Immunoprecipitation (IP)

Assay-Dependent

Product Specifications

Species Reactivity

Chicken, Human, Mouse, Non-human primate, Rat

Published species

Hamster, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Residues between 150 and 200 of human Glyceraldehyde-3-Phosphate Dehydrogenase (GeneID 2597)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

TBS with 0.1% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Wet ice

RRID

AB_568552

Product Specific Information

Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Suggested positive control: antigen standard for GAPDH (transient overexpression lysate).

Target Information

GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, GAPDH is ubiquitously expressed and displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. Studies provide evidence of GAPDH playing an essential part in gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases. Further, GAPDH is involved in other cellular processes ranging from membrane fusion, and neuronal apoptosis in cancer. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease (AD), and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations between GAPDH, actin and tubulin have also be reported. Since GAPDH is expressed at high levels in most tissues, it is useful as protein loading control in Western Blot analysis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

DYRK1A inhibitors leucettines and TGF-β inhibitor additively stimulate insulin production in beta cells, organoids, and isolated mouse islets.

PloS one

Pucelik B,Barzowska A,Czarna A

PA1-16777 was used in Western Blotting to show that leucettines, known to be DYRK1A kinase inhibitors, can improve glucose-stimulated insulin secretion (GSIS) in rodent β-cells and isolated islets, as well as in hiPSC-derived β-cells islets.

Fri May 19 00:00:00 EDT 2023

Absence of Scaffold Protein Tks4 Disrupts Several Signaling Pathways in Colon Cancer Cells.

International journal of molecular sciences

Jacksi M,Schad E,Buday L,Tantos A

PA1-16777 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to show a significant perturbation of gene expression upon the deletion of Tks4, suggesting the involvement of different signal transduction pathways over the well-known EGFR signaling.

Mon Jan 09 00:00:00 EST 2023

GDP polyribonucleotidyltransferase domain of vesicular stomatitis virus polymerase regulates leader-promoter escape and polyadenylation-coupled termination during stop-start transcription.

PLoS pathogens

Ogino M,Green TJ,Ogino T

PA1-16777 was used in Western Blot to study the functions of basic amino acid residues close to the vesicular stomatitis virus GDP polyribonucleotidyltransferase (PRNTase) active site in RNA biosynthesis, and identify two amino acid residues that regulate stop-start transcription at previously unappreciated steps.

Tue Feb 01 00:00:00 EST 2022

Detection of exogenous DNA uptake by murine dendritic cells.

STAR protocols

Celias DP,de Mingo Pulido Á,Ruffell B

PA1-16777 was used in Immunocytochemistry to measure exogenous DNA uptake by murine dendritic cells (DCs) using supernatant containing cellular debris, which allows for DNA uptake in the absence of transfection reagents.

Fri Sep 16 00:00:00 EDT 2022

Detection of exogenous DNA uptake by murine dendritic cells.

STAR protocols

Celias DP,de Mingo Pulido Á,Ruffell B

Fri Sep 16 00:00:00 EDT 2022

TRPM2 regulates autophagy to participate in hepatitis B virus replication.

Journal of viral hepatitis

Chen L,Zhu L,Lu X,Ming X,Yang B

PA1-16777 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to indicate that Hepatitis B virus (HVB) infection promotes TRPM2 expression, and TRPM2 interacts with p47 phox to induce autophagy and facilitate HVB replication.

Mon Aug 01 00:00:00 EDT 2022

MiR-30b-5p attenuates the inflammatory response and facilitates the functional recovery of spinal cord injury by targeting the NEFL/mTOR pathway.

Brain and behavior

Zhang H,Wang D,Tong J,Fang J,Lin Z

PA1-16777 was used in Western Blotting to investigate the role of Neurofilament Light Chain (NEFL) and its interaction with miR-30b-5p in spinal cord injury (SCI).

Thu Dec 01 00:00:00 EST 2022

Downregulation of circATXN7 represses non-small cell lung cancer growth by releasing miR-7-5p.

Thoracic cancer

Li D,Fu Z,Dong C,Song Y

PA1-16777 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to conclude that CircATXN7 accelerated the malignancy of NSCLC cells through adsorbing miR-7-5p and upregulating PFN2, offering evidence to support circATXN7 as a target for NSCLC treatment.

Wed Jun 01 00:00:00 EDT 2022

The inhibitory receptor TIM-3 limits activation of the cGAS-STING pathway in intra-tumoral dendritic cells by suppressing extracellular DNA uptake.

Immunity

de Mingo Pulido Á,Hänggi K,Celias DP,Gardner A,Li J,Batista-Bittencourt B,Mohamed E,Trillo-Tinoco J,Osunmakinde O,Peña R,Onimus A,Kaisho T,Kaufmann J,McEachern K,Soliman H,Luca VC,Rodriguez PC,Yu X,Ruffell B

PA1-16777 was used in Immunocytochemistry to report that increased CXCL9 expression by splenic cDC1s upon TIM-3 blockade required type I interferons and extracellular DNA.

Tue Jun 08 00:00:00 EDT 2021

Aberrant expression of semaphorin 6B affects cell phenotypes in thyroid carcinoma by activating the Notch signalling pathway.

Endokrynologia Polska

Lv XJ,Chen X,Wang Y,Yu S,Pang L,Huang C

PA1-16777 was used in Western Blot to demonstrate that SEMA6B exerts a tumourigenic effect in thyroid carcinoma partly by activating Notch signalling pathway, which provides a possible biomarker for the therapeutic intervention in thyroid carcinoma.

Wed Oct 20 00:00:00 EDT 2021

Activation of Cx43 Hemichannels Induces the Generation of Ca²⁺ Oscillations in White Adipocytes and Stimulates Lipolysis.

International journal of molecular sciences

Turovsky EA,Varlamova EG,Turovskaya MV

PA1-16777 was used in Western Blotting to investigate the mechanisms of Ca2+ oscillation generation upon activation of connexin-43 and regulation of the lipolysis/lipogenesis balance in white adipocytes through vesicular ATP release.

Wed Jul 28 00:00:00 EDT 2021

Genomic and epigenomic adaptation in SP-R210 (Myo18A) isoform-deficient macrophages.

Immunobiology

Yau E,Chen Y,Song C,Webb J,Carillo M,Kawasawa YI,Tang Z,Takahashi Y,Umstead TM,Dovat S,Chroneos ZC

PA1-16777 was used in Western Blot to suggest that the inflammatory differences seen in SP-R210L-deficient macrophages are a result of transcriptional differences that are mediated by epigenetic changes brought about by differential expression of the SP-R210 isoforms.

Mon Nov 01 00:00:00 EDT 2021

Novel compound mutations in the mitochondrial translation elongation factor (TSFM) gene cause severe cardiomyopathy with myocardial fibro-adipose replacement.

Scientific reports

Perli E,Pisano A,Glasgow RIC,Carbo M,Hardy SA,Falkous G,He L,Cerbelli B,Pignataro MG,Zacara E,Re F,Della Monica PL,Morea V,Bonnen PE,Taylor RW,d'Amati G,Giordano C

PA1-16777 was used in Western Blotting to report an unusual presentation of mitochondrial cardiomyopathy, with dilated phenotype and pathologic evidence of biventricular fibro-adipose replacement, in a 33-year old woman who underwent cardiac transplant.

Mon Mar 25 00:00:00 EDT 2019

Increased MIR31HG lncRNA expression increases gefitinib resistance in non-small cell lung cancer cell lines through the EGFR/PI3K/AKT signaling pathway.

Oncology letters

Wang B,Jiang H,Wang L,Chen X,Wu K,Zhang S,Ma S,Xia B

Published figure using GAPDH polyclonal antibody (Product # PA1-16777) in Western Blot

Mon May 01 00:00:00 EDT 2017

PAFAH1B1 and the lncRNA NONHSAT073641 maintain an angiogenic phenotype in human endothelial cells.

Acta physiologica (Oxford, England)

Josipovic I,Fork C,Preussner J,Prior KK,Iloska D,Vasconez AE,Labocha S,Angioni C,Thomas D,Ferreirós N,Looso M,Pullamsetti SS,Geisslinger G,Steinhilber D,Brandes RP,Leisegang MS

PA1-16777 was used in western blot to analyze maintenance of an angiogenic phenotype in human endothelial cells by PAFAH1B1 and the IncRNA NONHSAT073641

Thu Sep 01 00:00:00 EDT 2016

Characterization of metastatic tumor antigen 1 and its interaction with hepatitis B virus X protein in NF-κB signaling and tumor progression in a woodchuck hepatocellular carcinoma model.

Oncotarget

Li YT,Liu CJ,Su TH,Cheng HR,Jeng YM,Lin HL,Wang CC,Kao JH,Chen PJ,Chen DS,Wu HL

PA116777 was used in western blot to explore the role of metastatic tumor antigen 1 in hepatitis B-associated hepatocarcinogenesis in the woodchuck model

Tue Jul 26 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: 38 kDa BFA-dependent ADP-ribosylation substrate; aging-associated gene 9 protein; BARS-38; EC 1.2.1.12; epididymis secretory protein Li 278; epididymis secretory sperm binding protein Li 162eP; GAPDH; GAPDH-2; glceraldehyde-3-phosphate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase type 2; Glyceraldehyde-3-phosphate dehydrogenase, testis-specific; HGNC:4141; MGC88685; multifunctional protein, glycolytic enzyme; Peptidyl-cysteine S-nitrosylase GAPDH; Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2; Spermatogenic glyceraldehyde-3-phosphate dehydrogenase; unnamed protein product

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Gene Aliases: BARS-38; CDABP0047; G3PD; GAPD; Gapd-s; GAPD2; GAPDH; GAPDH-2; GAPDH2; GAPDHS; GAPDS; HEL-S-162eP; HEL-S-278; HSD-35; HSD35; KNC-NDS6; OK/SW-cl.12

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UniProt ID: (Human) P04406, (Chicken) P00356, (Human) O14556, (Mouse) P16858, (Rat) P04797, (Rat) Q9ESV6, (Mouse) Q64467

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Entrez Gene ID: (Human) 2597, (Chicken) 374193, (Human) 26330, (Mouse) 14433, (Rat) 24383, (Rat) 66020, (Mouse) 14447

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Function(s)

glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity protein binding microtubule binding peptidyl-cysteine S-nitrosylase activity identical protein binding NADP binding NAD binding aspartic-type endopeptidase inhibitor activity disordered domain specific binding molecular_function oxidoreductase activity oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor dehydrogenase

Process(es)

microtubule cytoskeleton organization gluconeogenesis regulation of macroautophagy negative regulation of translation peptidyl-cysteine S-trans-nitrosylation protein stabilization neuron apoptotic process canonical glycolysis cellular response to interferon-gamma nuclear membrane fusion glycolytic process negative regulation of endopeptidase activity negative regulation of apoptotic process positive regulation of cytokine secretion defense response to fungus killing by host of symbiont cells positive regulation by organism of apoptotic process in other organism involved in symbiotic interaction oxidation-reduction process antimicrobial humoral immune response mediated by antimicrobial peptide spermatid development sperm motility positive regulation of glycolytic process carbohydrate metabolic process apoptotic process multicellular organism development response to ammonium ion regulation of translation glucose metabolic process

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GAPDH Polyclonal Antibody

GAPDH Antibody (PA1-16777) in ICC/IF

Product Details

PA1-16777

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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