Invitrogen

GAPDH Polyclonal Antibody

2 Published Figures

3 References

View all (122) GAPDH antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (9)
Published Figures (2)

GAPDH Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 11

GAPDH Antibody (PA5-85074) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of GAPDH was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: GAPDH Polyclonal Antibody (Product # PA5-85074) diluted at 1:2000. Blue: Hoechst 33342 staining.

GAPDH Antibody in Immunohistochemistry (Paraffin) (IHC (P))

GAPDH Antibody in Immunoprecipitation (IP)

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Product Details

PA5-85074

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000-1:100,000

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Immunoprecipitation (IP)

1:100-1:500

Product Specifications

Species Reactivity

Bovine, Dog, Fruit fly, Hamster, Human, Mouse, Non-human primate, Pig, Rabbit, Rat, Zebrafish

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human GAPDH.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.08 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2792223

Product Specific Information

Keep as concentrated solution.

Predicted reactivity: Mouse (94%), Rat (94%), Zebrafish (86%), Xenopus laevis (85%), Dog (95%), Cat (96%), Pig (95%), Rabbit (94%), Chicken (92%), Sheep (94%), Rhesus Monkey (99%), Bovine (94%), Guinea pig (94%).

Positive Control: 293T, A431, HeLa, HepG2, Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12, Rat2, E.coli M15, SH-SY5Y, A549, U2OS, H9C2, A7R5, mouse skin tissue.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, GAPDH is ubiquitously expressed and displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. Studies provide evidence of GAPDH playing an essential part in gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases. Further, GAPDH is involved in other cellular processes ranging from membrane fusion, and neuronal apoptosis in cancer. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease (AD), and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations between GAPDH, actin and tubulin have also be reported. Since GAPDH is expressed at high levels in most tissues, it is useful as protein loading control in Western Blot analysis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Tubulin tyrosination regulates synaptic function and is disrupted in Alzheimer's disease.

Brain : a journal of neurology

Peris L,Parato J,Qu X,Soleilhac JM,Lanté F,Kumar A,Pero ME,Martínez-Hernández J,Corrao C,Falivelli G,Payet F,Gory-Fauré S,Bosc C,Blanca Ramirez M,Sproul A,Brocard J,Di Cara B,Delagrange P,Buisson A,Goldberg Y,Moutin MJ,Bartolini F,Andrieux A

PA5-85074 was used in Western Blot to demonstrate that a balanced tyrosination/detyrosination tubulin cycle is necessary for the maintenance of synaptic plasticity, is protective against amyloid β peptide-induced synaptic damage, and that this balance is lost in Alzheimer's disease, providing evidence that defective tubulin retyrosination may contribute to circuit dysfunction during neurodegeneration in Alzheimer's disease.

Fri Jul 29 00:00:00 EDT 2022

RAF1 mediates the FSH signaling pathway as a downstream molecule to stimulate estradiol synthesis and secretion in mouse ovarian granulosa cells.

Annals of translational medicine

Luo X,Liu H,Guo H,Sun L,Gou K,Cui S

Published figure using GAPDH polyclonal antibody (Product # PA5-85074) in Western Blot

Tue Mar 01 00:00:00 EST 2022

Hsa_circ_0074032 promotes prostate cancer progression through elevating homeobox A1 expression by serving as a microRNA-198 decoy.

Andrologia

Feng C,Wang Q,Deng L,Peng N,Yang M,Wang X

PA5-85074 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to demonstrate that high circ_0074032 level in PCa is associated with worse PCa-related prognosis. Circ_0074032 induced cell apoptosis, curbed cell proliferation, migration and invasion in PCa cells and was identified as miR-198 decoy. Circ_0074032 regulates HOXA1 expression and promotes PCa progression via elevating HOXA1 expression through acting as a miR-198 sponge.

Tue Feb 01 00:00:00 EST 2022

Bioinformatics

Protein Aliases: 38 kDa BFA-dependent ADP-ribosylation substrate; aging-associated gene 9 protein; BARS-38; EC 1.2.1.12; epididymis secretory protein Li 278; epididymis secretory sperm binding protein Li 162eP; GAPD; GAPDH; GAPDH-2; glyceraldehyde 3-phosphate dehydrogenase, testis-specific; glyceraldehyde phosphate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase 2; glyceraldehyde-3-phosphate dehydrogenase type 2; glyceraldehyde-3-phosphate dehydrogenase, spermatogenic; Glyceraldehyde-3-phosphate dehydrogenase, testis-specific; HGNC:4141; MGC88685; Peptidyl-cysteine S-nitrosylase GAPDH; Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2; Spermatogenic glyceraldehyde-3-phosphate dehydrogenase; unnamed protein product

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Gene Aliases: BARS-38; bb02e05; cb350; cb609; CDABP0047; fb71f08; fk58c09; G3PD; g3pdh; GAPD; Gapd-s; GAPD2; GAPDH; GAPDH-2; GAPDH2; GAPDHS; GAPDS; HEL-S-162eP; HEL-S-278; HSD-35; HSD35; I79_001391; mg:bb02e05; OK/SW-cl.12; wu:fb33a10; wu:fb71f08; wu:fk58c09; wu:ft80f05; zgc:76908

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UniProt ID: (Rabbit) P46406, (Human) P04406, (Pig) P00355, (Bovine) P10096, (Human) O14556, (Bovine) Q2KJE5, (Mouse) P16858, (Chinese hamster) P17244, (Rat) P04797, (Rat) Q9ESV6, (Mouse) Q64467, (Zebrafish) Q5XJ10, (Zebrafish) Q5MJ86

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Function(s)

glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity protein binding microtubule binding peptidyl-cysteine S-nitrosylase activity identical protein binding NADP binding NAD binding disordered domain specific binding molecular_function oxidoreductase activity oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor transferase activity nucleotide binding dehydrogenase

Process(es)

microtubule cytoskeleton organization gluconeogenesis regulation of macroautophagy negative regulation of translation peptidyl-cysteine S-trans-nitrosylation protein stabilization neuron apoptotic process canonical glycolysis cellular response to interferon-gamma glucose metabolic process glycolytic process regulation of translation oxidation-reduction process regulation of neurotransmitter loading into synaptic vesicle spermatid development sperm motility positive regulation of glycolytic process carbohydrate metabolic process apoptotic process multicellular organism development response to ammonium ion

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GAPDH Polyclonal Antibody

GAPDH Antibody (PA5-85074) in ICC/IF

Product Details

PA5-85074

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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