Invitrogen

GAPDH Polyclonal Antibody

20 Published Figures

22 References

View all (122) GAPDH antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (20)

FIGURE: 1 / 21

GAPDH Antibody (TAB1001) in WB

Western blot analysis of GAPDH using a polyclonal antibody (Product # TAB1001).

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Product Details

TAB1001

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5 µg/mL

View 22 publications 22 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated peptide representing human GAPDH

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

BBS, pH 8.4

Contains

no preservative

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10709707

Product Specific Information

TAB1001 detects GAPDH in samples.

TAB1001 has been successfully used in Western Blot applications.

The TAB1001 immunogen is a KLH conjugated peptide representing human GAPDH.

Target Information

GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, GAPDH is ubiquitously expressed and displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. Studies provide evidence of GAPDH playing an essential part in gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases. Further, GAPDH is involved in other cellular processes ranging from membrane fusion, and neuronal apoptosis in cancer. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease (AD), and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations between GAPDH, actin and tubulin have also be reported. Since GAPDH is expressed at high levels in most tissues, it is useful as protein loading control in Western Blot analysis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A Helicase Unwinds Hexanucleotide Repeat RNA G-Quadruplexes and Facilitates Repeat-Associated Non-AUG Translation.

Journal of the American Chemical Society

Liu H,Lu YN,Paul T,Periz G,Banco MT,Ferré-D'Amaré AR,Rothstein JD,Hayes LR,Myong S,Wang J

TAB1001 was used in Western Blotting to indicate that DHX36 facilitates C9orf72 RAN translation by resolving repeat RNA G-quadruplex structures and may be a potential target for therapeutic intervention.

Wed May 19 00:00:00 EDT 2021

Implications of cell division cycle associated 4 on the Wilm's tumor cells viability via AKT/mTOR signaling pathway.

Renal failure

Li S,Qin C,Chen Y,Wei D,Tan Z,Meng J

Published figure using GAPDH polyclonal antibody (Product # TAB1001) in Western Blot

Wed Dec 01 00:00:00 EST 2021

G-quadruplexes offer a conserved structural motif for NONO recruitment to NEAT1 architectural lncRNA.

Nucleic acids research

Simko EAJ,Liu H,Zhang T,Velasquez A,Teli S,Haeusler AR,Wang J

Published figure using GAPDH polyclonal antibody (Product # TAB1001) in Western Blot

Mon Jul 27 00:00:00 EDT 2020

Tetraspanin CD82 is necessary for muscle stem cell activation and supports dystrophic muscle function.

Skeletal muscle

Hall A,Fontelonga T,Wright A,Bugda Gwilt K,Widrick J,Pasut A,Villa F,Miranti CK,Gibbs D,Jiang E,Meng H,Lawlor MW,Gussoni E

TAB1001 was used in Western Blotting to study the consequences of loss of CD82 expression in normal and dystrophic skeletal muscle and examined the dysregulation of downstream functions in mice aged up to 1 year.

Fri Nov 27 00:00:00 EST 2020

Tetraspanin CD82 is necessary for muscle stem cell activation and supports dystrophic muscle function.

Skeletal muscle

Hall A,Fontelonga T,Wright A,Bugda Gwilt K,Widrick J,Pasut A,Villa F,Miranti CK,Gibbs D,Jiang E,Meng H,Lawlor MW,Gussoni E

Fri Nov 27 00:00:00 EST 2020

Molecular determinants of SR-B1-dependent Plasmodium sporozoite entry into hepatocytes.

Scientific reports

Langlois AC,Manzoni G,Vincensini L,Coppée R,Marinach C,Guérin M,Huby T,Carrière V,Cosset FL,Dreux M,Rubinstein E,Silvie O

Published figure using GAPDH polyclonal antibody (Product # TAB1001) in Western Blot

Tue Aug 11 00:00:00 EDT 2020

Targeting viperin to the mitochondrion inhibits the thiolase activity of the trifunctional enzyme complex.

The Journal of biological chemistry

Dumbrepatil AB,Zegalia KA,Sajja K,Kennedy RT,Marsh ENG

TAB1001 was used in Western Blot to provide biochemical insight into the mechanism by which human cytomegalovirus subverts viperin.

Fri Feb 28 00:00:00 EST 2020

L3MBTL1 regulates ALS/FTD-associated proteotoxicity and quality control.

Nature neuroscience

Lu J,Periz G,Lu YN,Tang Q,Liu Y,Zhang T,Shah Y,Thombre R,Aljumaah R,Li W,Mojsilovic-Petrovic J,Ji Y,Johnson K,Kalb R,Wang J

Published figure using GAPDH polyclonal antibody (Product # TAB1001) in Western Blot

Sat Jun 01 00:00:00 EDT 2019

Tumor Necrosis Factor-α-Mediated Metaplastic Inhibition of LTP Is Constitutively Engaged in an Alzheimer's Disease Model.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Singh A,Jones OD,Mockett BG,Ohline SM,Abraham WC

TAB1001 was used in Western Blotting to there is an endogenous engagement of the metaplasticity mechanism in this mouse model of AD, supporting the idea that blocking TNFα might be of therapeutic benefit in the disease.

Wed Nov 13 00:00:00 EST 2019

miR‑378a‑3p inhibits cellular proliferation and migration in glioblastoma multiforme by targeting tetraspanin 17.

Oncology reports

Guo XB,Zhang XC,Chen P,Ma LM,Shen ZQ

TAB1001 was used in Western Blotting to highlight that the progression of glioblastoma multiforme is supressed by miR-378a-3p through reduced tetraspanin 17 expression.

Fri Nov 01 00:00:00 EDT 2019

A novel null mutation in the pyruvate dehydrogenase phosphatase catalytic subunit gene (PDP1) causing pyruvate dehydrogenase complex deficiency.

JIMD reports

Bedoyan JK,Hecht L,Zhang S,Tarrant S,Bergin A,Demirbas D,Yang E,Shin HK,Grahame GJ,DeBrosse SD,Hoppel CL,Kerr DS,Berry GT

TAB1001 was used in Western Blotting to show the fourth case of functional pyruvate dehydrogenase complex deficiency with a defined mutation in PDP1.

Mon Jul 01 00:00:00 EDT 2019

Viperin interacts with the kinase IRAK1 and the E3 ubiquitin ligase TRAF6, coupling innate immune signaling to antiviral ribonucleotide synthesis.

The Journal of biological chemistry

Dumbrepatil AB,Ghosh S,Zegalia KA,Malec PA,Hoff JD,Kennedy RT,Marsh ENG

TAB1001 was used in Western Blotting to conclude that the synergistic activation of viperin and IRAK1 provides a mechanism that couples innate immune signaling with the production of the antiviral nucleotide ddhCTP.

Fri Apr 26 00:00:00 EDT 2019

Sumoylation promotes optimal APC/C Activation and Timely Anaphase.

eLife

Lee CC,Li B,Yu H,Matunis MJ

Published figure using GAPDH polyclonal antibody (Product # TAB1001) in Western Blot

Thu Mar 08 00:00:00 EST 2018

HSP90 stabilizes B-cell receptor kinases in a multi-client interactome: PU-H71 induces CLL apoptosis in a cytoprotective microenvironment.

Oncogene

Guo A,Lu P,Lee J,Zhen C,Chiosis G,Wang YL

TAB1001 was used in Western Blotting to study the role of HSP90 in causing B-cell receptor over-activity in chronic lymphocytic leukaemia.

Thu Jun 15 00:00:00 EDT 2017

Rapamycin rescues vascular, metabolic and learning deficits in apolipoprotein E4 transgenic mice with pre-symptomatic Alzheimer's disease.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism

Lin AL,Jahrling JB,Zhang W,DeRosa N,Bakshi V,Romero P,Galvan V,Richardson A

TAB1001 was used in western blot to test if early intervention with rapamycin restores neurovascular and neurometabolic functions in a murine model of Alzheimer's disease

Sun Jan 01 00:00:00 EST 2017

BET bromodomain inhibitors and agonists of the beta-2 adrenergic receptor identified in screens for compounds that inhibit DUX4 expression in FSHD muscle cells.

Skeletal muscle

Campbell AE,Oliva J,Yates MP,Zhong JW,Shadle SC,Snider L,Singh N,Tai S,Hiramuki Y,Tawil R,van der Maarel SM,Tapscott SJ,Sverdrup FM

TAB1001 was used in Western Blotting to identify molecules that decrease double homeobox 4 expression in facioscapulohumeral dystrophy patients.

Mon Sep 04 00:00:00 EDT 2017

MeCP2 Affects Skeletal Muscle Growth and Morphology through Non Cell-Autonomous Mechanisms.

PloS one

Conti V,Gandaglia A,Galli F,Tirone M,Bellini E,Campana L,Kilstrup-Nielsen C,Rovere-Querini P,Brunelli S,Landsberger N

TAB1001 was used in western blot to study the muscular defects in Mecp2-null mice.

Mon Apr 25 00:00:00 EDT 2016

The SRA protein UHRF1 promotes epigenetic crosstalks and is involved in prostate cancer progression.

Oncogene

Babbio F,Pistore C,Curti L,Castiglioni I,Kunderfranco P,Brino L,Oudet P,Seiler R,Thalman GN,Roggero E,Sarti M,Pinton S,Mello-Grand M,Chiorino G,Catapano CV,Carbone GM,Bonapace IM

TAB1001 was used in western blot to study the role of UHRF1 in epigenetic cross-talk and prostate cancer progression

Thu Nov 15 00:00:00 EST 2012

Analysis of the myosin-II-responsive focal adhesion proteome reveals a role for β-Pix in negative regulation of focal adhesion maturation.

Nature cell biology

Kuo JC,Han X,Hsiao CT,Yates JR,Waterman CM

TAB1001 was used in western blot to study the role of myosin II activity in focal adhesion using proteomics

Fri Apr 01 00:00:00 EDT 2011

Activated Met signalling in the developing mouse heart leads to cardiac disease.

PloS one

Leo C,Sala V,Morello M,Chiribiri A,Riess I,Mancardi D,Schiaffino S,Ponzetto C,Crepaldi T

Published figure using GAPDH polyclonal antibody (Product # TAB1001) in Western Blot

Wed Feb 09 00:00:00 EST 2011

Long-range transcriptional control of progesterone receptor gene expression.

Molecular endocrinology (Baltimore, Md.)

Bonéy-Montoya J,Ziegler YS,Curtis CD,Montoya JA,Nardulli AM

TAB1001 was used in western blot to investigate the transcriptional regulation of progesterone receptor gene

Mon Feb 01 00:00:00 EST 2010

Thioredoxin and thioredoxin reductase influence estrogen receptor alpha-mediated gene expression in human breast cancer cells.

Journal of molecular endocrinology

Rao AK,Ziegler YS,McLeod IX,Yates JR,Nardulli AM

TAB1001 was used in western blot to investigate the effect of thioredoxin and thioredoxin reductase on gene transcription in breast tumors

Tue Dec 01 00:00:00 EST 2009

Apurinic/apyrimidinic endonuclease 1 alters estrogen receptor activity and estrogen-responsive gene expression.

Molecular endocrinology (Baltimore, Md.)

Curtis CD,Thorngren DL,Ziegler YS,Sarkeshik A,Yates JR,Nardulli AM

TAB1001 was used in western blot to investigate the influenece of apurinic/apyrimidinic endonuclease 1 on estrogen receptor activity and related gene expression

Tue Sep 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: aging-associated gene 9 protein; EC 1.2.1.12; epididymis secretory protein Li 278; epididymis secretory sperm binding protein Li 162eP; GAPDH; GAPDH-2; Glyceraldehyde-3-phosphate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase, testis-specific; HGNC:4141; MGC88685; Peptidyl-cysteine S-nitrosylase GAPDH; Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2; Spermatogenic glyceraldehyde-3-phosphate dehydrogenase

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Gene Aliases: CDABP0047; G3PD; GAPD; GAPD2; GAPDH; GAPDH-2; GAPDH2; GAPDHS; GAPDS; HEL-S-162eP; HEL-S-278; HSD-35; HSD35; OK/SW-cl.12

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UniProt ID: (Human) P04406, (Human) O14556

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Entrez Gene ID: (Human) 2597, (Human) 26330

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Function(s)

glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity protein binding microtubule binding peptidyl-cysteine S-nitrosylase activity identical protein binding NADP binding NAD binding dehydrogenase

Process(es)

microtubule cytoskeleton organization gluconeogenesis regulation of macroautophagy negative regulation of translation peptidyl-cysteine S-trans-nitrosylation protein stabilization neuron apoptotic process canonical glycolysis cellular response to interferon-gamma spermatid development sperm motility positive regulation of glycolytic process

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GAPDH Polyclonal Antibody

GAPDH Antibody (TAB1001) in WB

Product Details

TAB1001

Western Blot (WB)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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