Invitrogen

GAPDH Monoclonal Antibody (258)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

10 Published Figures

26 References

View all (122) GAPDH antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

GAPDH Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 15

GAPDH Antibody (437000) in ICC/IF

Immunofluorescent analysis of GAPDH was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with GAPDH Mouse Monoclonal Antibody (Product # 437000) at 1 µg/mL and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Rabbit Anti-Mouse IgG Secondary Antibody (Product # A-11059) at a dilution of 1:400 for 30 minutes at room tempera... View More

GAPDH Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

GAPDH Antibody — Identification of Iron Chelators as a Class of shRNA-Mediated RNAi Enhancer via Chemical Screen (A) Iron chelators enhance shRNA-EGFP-mediated gene silencing. DMSO was used as a negative control, whereas enoxacin, a previously identified RNAi enhancer, was used as a positive control. Values are mean ± SD for triplicate samples. *p < 0.001 when metal chelators were compared with control or enoxacin, and enoxacin was compared with control. The left panel shows the relative GFP/RFP ratios, while the right panel shows the GFP mRNA level determined by quantitative RT-PCR. Values are mean ± SD for triplicate samples. (B) The metal chelator 2,2'-dipyridyl enhances shGAPDH-mediated gene silencing. The GAPDH protein levels were detected by western blots with anti-GAPDH antibody, with Spectrin used as a loading control. Metal chelator has no effect on GAPDH expression in shVC control cells. Values are mean ± SD for triplicate samples. *p < 0.001 when shGAPDH treated with 2,2'-dipyridyl was compared with shGAPDH control, and shGAPDH control was compared with shVC. (C) The iron chelator 2,2'-dipyridyl, but not its inactive isomer 4,4'-bipyridyl, could enhance shRNA-mediated RNAi. Three reporter cell lines, shGFP, shLuciferase, and shGAPDH, were treated with 2,2'-dipyridyl and its isoform 4,4'-bipyridyl for 48 hr before analyses. Values are mean ± SD for triplicate samples. *p < 0.001 when 2,2'-dipyridyl was compared with control (DMSO) and 4,4'-bipyridyl. (D) Iron specifically regulates {KD}

Product Details

437000

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 22 publications 22 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunocytochemistry (ICC/IF)

1-2 µg/mL

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Bovine, Horse, Cat, Human, Non-human primate, Sheep, Pig

Published species

Human, Mouse, Rhesus monkey

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

258

Immunogen

Recombinant protein derived from the C-terminus region of human GAPDH protein

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10374327

Target Information

GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, GAPDH is ubiquitously expressed and displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. Studies provide evidence of GAPDH playing an essential part in gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases. Further, GAPDH is involved in other cellular processes ranging from membrane fusion, and neuronal apoptosis in cancer. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease (AD), and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations between GAPDH, actin and tubulin have also be reported. Since GAPDH is expressed at high levels in most tissues, it is useful as protein loading control in Western Blot analysis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

MicroRNA-650 Regulates the Pathogenesis of Alzheimer's Disease Through Targeting Cyclin-Dependent Kinase 5.

Molecular neurobiology

Lin L,Liu X,Cheng X,Li Y,Gearing M,Levey A,Huang X,Li Y,Jin P,Li X

437000 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to show that miR-650 is able to significantly reduce the expression of APOE, PSEN1, and CDK5 in vitro and significantly alter the CDK5 level and ameliorate AD pathologies in APP-PSEN1 transgenic mice.

Mon May 01 00:00:00 EDT 2023

Molecular Mechanisms of the Cytotoxic Effect of Recombinant Selenoprotein SELENOM on Human Glioblastoma Cells.

International journal of molecular sciences

Rogachev VV,Goltyaev MV,Varlamova EG,Turovsky EA

437000 was used in Western Blot to demonstrate a dose-dependent depletion of the Ca2+ pool under the action of SELENOM, which proves the important role of this protein in the regulation of calcium homeostasis in the cell.

Thu Mar 30 00:00:00 EDT 2023

The Role of Selenoproteins SELENOM and SELENOT in the Regulation of Apoptosis, ER Stress, and Calcium Homeostasis in the A-172 Human Glioblastoma Cell Line.

Biology

Varlamova EG,Goltyaev MV,Turovsky EA

437000 was used in Western Blotting to investigate the mechanisms of fine regulation of the processes of apoptosis, cell proliferation, and ER stress by two ER resident proteins, SELENOM and SELENOT.

Wed May 25 00:00:00 EDT 2022

MicroRNA-223 Suppresses Human Hepatic Stellate Cell Activation Partly via Regulating the Actin Cytoskeleton and Alleviates Fibrosis in Organoid Models of Liver Injury.

International journal of molecular sciences

Ariyachet C,Chuaypen N,Kaewsapsak P,Chantaravisoot N,Jindatip D,Potikanond S,Tangkijvanich P

437000 was used in Western Blotting to show that ectopic expression of miR-223 in hepatic stellate cells attenuated fibrogenesis in the two human organoid models of liver injury, suggesting its potential application in antifibrotic therapy.

Fri Aug 19 00:00:00 EDT 2022

Comparative Analysis of the Cytotoxic Effect of a Complex of Selenium Nanoparticles Doped with Sorafenib, "Naked" Selenium Nanoparticles, and Sorafenib on Human Hepatocyte Carcinoma HepG2 Cells.

International journal of molecular sciences

Varlamova EG,Goltyaev MV,Simakin AV,Gudkov SV,Turovsky EA

437000 was used in Western Blotting to suggest that our data on the mechanisms of action and the created nanocomplex are promising as a platform for the creation of highly selective and effective drugs with targeted delivery to tumors.

Tue Jun 14 00:00:00 EDT 2022

A deep redox proteome profiling workflow and its application to skeletal muscle of a Duchenne Muscular Dystrophy model.

Free radical biology & medicine

Day NJ,Zhang T,Gaffrey MJ,Zhao R,Fillmore TL,Moore RJ,Rodney GG,Qian WJ

437000 was used in Western Blot to report a deep redox proteome profiling workflow and demonstrate its application in measuring the changes in thiol oxidation along with global protein expression in skeletal muscle from mdx mice, a model of Duchenne Muscular Dystrophy (DMD).

Sun Nov 20 00:00:00 EST 2022

Laminar Flow on Endothelial Cells Suppresses eNOS O-GlcNAcylation to Promote eNOS Activity.

Circulation research

Basehore SE,Bohlman S,Weber C,Swaminathan S,Zhang Y,Jang C,Arany Z,Clyne AM

437000 was used in Western Blotting to test if steady laminar flow, but not oscillating disturbed flow, decreases eNOS O-GlcNAcylation, thereby elevating eNOS phosphorylation and NO production.

Fri Nov 12 00:00:00 EST 2021

Laminar Flow on Endothelial Cells Suppresses eNOS O-GlcNAcylation to Promote eNOS Activity.

Circulation research

Basehore SE,Bohlman S,Weber C,Swaminathan S,Zhang Y,Jang C,Arany Z,Clyne AM

437000 was used in Western Blotting to test if steady laminar flow, but not oscillating disturbed flow, decreases eNOS O-GlcNAcylation, thereby elevating eNOS phosphorylation and NO production.

Fri Nov 12 00:00:00 EST 2021

Human Adipose-derived mesenchymal stem cells promote lymphocyte apoptosis and alleviate atherosclerosis via miR-125b-1-3p/BCL11B signal axis.

Annals of palliative medicine

Yu C,Tang W,Lu R,Tao Y,Ren T,Gao Y

437000 was used in Western Blot to show that miR-125b-1-3p was expressed in hMSCs-Ad exosomes and can promote T lymphocyte apoptosis and alleviate AS by down-regulating BCL11B expression.

Mon Feb 01 00:00:00 EST 2021

High-throughput screening for natural compound-based autophagy modulators reveals novel chemotherapeutic mode of action for arzanol.

Cell death & disease

Deitersen J,Berning L,Stuhldreier F,Ceccacci S,Schlütermann D,Friedrich A,Wu W,Sun Y,Böhler P,Berleth N,Mendiburo MJ,Seggewiß S,Anand R,Reichert AS,Monti MC,Proksch P,Stork B

437000 was used in Western Blot to search for autophagy-modulating compounds for possible cancer therapy using flow cytometry-based high-throughput screening.

Mon May 31 00:00:00 EDT 2021

Transgelin-2 and phosphoregulation of the LIC2 subunit of dynein govern mitotic spindle orientation.

Journal of cell science

Sharma A,Dagar S,Mylavarapu SVS

437000 was used in Western Blotting to discover how spindle orientation is regulated by transgelin-2 engagement of LGN-NuMA and mitotic phosphoregulation of the LIC2 subunit of dynein.

Tue Jun 23 00:00:00 EDT 2020

HIFα independent mechanisms in renal carcinoma cells modulate divergent outcomes in fibronectin assembly mediated by hypoxia and CoCl₂.

Scientific reports

Magdaleno C,Dixon L,Rajasekaran N,Varadaraj A

437000 was used in Immunocytochemistry-immunoflourescence to systematically reexamine the role of hypoxia mimetics as experimental substitutes for hypoxia and provides new findings on HIFα stabilization and the FN matrix in the context of renal cancer.

Thu Oct 29 00:00:00 EDT 2020

HIFα independent mechanisms in renal carcinoma cells modulate divergent outcomes in fibronectin assembly mediated by hypoxia and CoCl₂.

Scientific reports

Magdaleno C,Dixon L,Rajasekaran N,Varadaraj A

Thu Oct 29 00:00:00 EDT 2020

Biomechanical regulation of drug sensitivity in an engineered model of human tumor.

Biomaterials

Marturano-Kruik A,Villasante A,Yaeger K,Ambati SR,Chramiec A,Raimondi MT,Vunjak-Novakovic G

437000 was used in Western Blotting to study the effects of mechanical signals on cancer cell survival and drug sensitivity.

Mon Jan 01 00:00:00 EST 2018

Selenium supplementation through Se-rich dietary matrices can upregulate the anti-inflammatory responses in lipopolysaccharide-stimulated murine macrophages.

Food and agricultural immunology

Dhanjal NIK,Sharma S,Prabhu KS,Prakash NT

437000 was used in Western Blotting to demonstrate that SeSup effectively modulates the expression inflammatory responses, indicating the potential benefits of dietary selenium supplementation.

Thu Oct 01 00:00:00 EDT 2020

The addition of abemaciclib to sunitinib induces regression of renal cell carcinoma xenograft tumors.

Oncotarget

Small J,Washburn E,Millington K,Zhu J,Holder SL

437000 was used in Western Blotting to show that abemaciclib in combination with sunitinib causes dramatic reduction in tumour sizes without overt toxicity in a mouse model of renal cell carcinoma.

Fri Nov 10 00:00:00 EST 2017

Vascularized Tissue-Engineered Model for Studying Drug Resistance in Neuroblastoma.

Theranostics

Villasante A,Sakaguchi K,Kim J,Cheung NK,Nakayama M,Parsa H,Okano T,Shimizu T,Vunjak-Novakovic G

Published figure using GAPDH monoclonal antibody (Product # 437000) in Western Blot

Wed Jun 27 00:00:00 EDT 2018

Recapitulating the Size and Cargo of Tumor Exosomes in a Tissue-Engineered Model.

Theranostics

Villasante A,Marturano-Kruik A,Ambati SR,Liu Z,Godier-Furnemont A,Parsa H,Lee BW,Moore MA,Vunjak-Novakovic G

437000 was used in Western Blotting to provide evidence that an in vitro culture environment can recapitulate some properties of tumor exosomes.

Mon Oct 16 00:00:00 EDT 2017

Actin remodeling by Nck regulates endothelial lumen formation.

Molecular biology of the cell

Chaki SP,Barhoumi R,Rivera GM

437000 was used in western blot to study how Nck signaling contributes to vascular formation and remodeling

Tue Sep 01 00:00:00 EDT 2015

Quantitative proteomics of synaptic and nonsynaptic mitochondria: insights for synaptic mitochondrial vulnerability.

Journal of proteome research

Stauch KL,Purnell PR,Fox HS

437000 was used in western blot to study the susceptibility of synaptic mitochondria to damage.

Fri May 02 00:00:00 EDT 2014

Quantitative proteomics reveals oxygen-dependent changes in neuronal mitochondria affecting function and sensitivity to rotenone.

Journal of proteome research

Villeneuve L,Tiede LM,Morsey B,Fox HS

437000 was used in western blot to examine the impact of oxygen levels on mitochondrial function in neurons.

Fri Oct 04 00:00:00 EDT 2013

Nck enables directional cell migration through the coordination of polarized membrane protrusion with adhesion dynamics.

Journal of cell science

Chaki SP,Barhoumi R,Berginski ME,Sreenivasappa H,Trache A,Gomez SM,Rivera GM

437000 was used in western blot to elucidate the role of Nck in cytoskeletal remodeling during directional migration.

Mon Apr 01 00:00:00 EDT 2013

Autophagy-mediated turnover of dynamin-related protein 1.

BMC neuroscience

Purnell PR,Fox HS

Published figure using GAPDH monoclonal antibody (Product # 437000) in Western Blot

Fri Aug 09 00:00:00 EDT 2013

Large protein complexes retained in the ER are dislocated by non-COPII vesicles and degraded by selective autophagy.

Cellular and molecular life sciences : CMLS

Le Fourn V,Park S,Jang I,Gaplovska-Kysela K,Guhl B,Lee Y,Cho JW,Zuber C,Roth J

437000 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to demonstrate that different pathways exist in parallel for ER-to-cytoplasm dislocation and subsequent proteolytic degradation of large luminal protein complexes and of surplus luminal single-chain proteins.

Sat Jun 01 00:00:00 EDT 2013

Iron homeostasis regulates the activity of the microRNA pathway through poly(C)-binding protein 2.

Cell metabolism

Li Y,Lin L,Li Z,Ye X,Xiong K,Aryal B,Xu Z,Paroo Z,Liu Q,He C,Jin P

Published figure using GAPDH monoclonal antibody (Product # 437000) in Western Blot

Wed Jun 06 00:00:00 EDT 2012

A quantitative TR-FRET plate reader immunoassay for measuring autophagy.

Autophagy

Hancock MK,Hermanson SB,Dolman NJ

437000 was used in western blot to develop a TR-FRET immuno-assay for the quantitative measurement of autophagy using GFP-tagged LC3B.

Wed Aug 01 00:00:00 EDT 2012

Membrane fusion-mediated autophagy induction enhances morbillivirus cell-to-cell spread.

Journal of virology

Delpeut S,Rudd PA,Labonté P,von Messling V

437000 was used in western blot to report that morbilliviruses rapidly induce autophagy for efficient cell-to-cell spread.

Wed Aug 01 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: aging-associated gene 9 protein; EC 1.2.1.12; epididymis secretory protein Li 278; epididymis secretory sperm binding protein Li 162eP; GAPD; GAPDH; GAPDH-2; glyceraldehyde phosphate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase, testis-specific; HGNC:4141; MGC88685; Peptidyl-cysteine S-nitrosylase GAPDH; Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2; Spermatogenic glyceraldehyde-3-phosphate dehydrogenase

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Gene Aliases: CDABP0047; G3PD; G3PDH; GAPD; GAPD2; GAPDH; GAPDH-2; GAPDH2; GAPDHS; GAPDS; HEL-S-162eP; HEL-S-278; HSD-35; HSD35; OK/SW-cl.12

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UniProt ID: (Human) P04406, (Bovine) P10096, (Cat) Q9N2D5, (Pig) P00355, (Human) O14556, (Bovine) Q2KJE5

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Entrez Gene ID: (Sheep) 443005, (Human) 2597, (Bovine) 281181, (Cat) 493876, (Horse) 100033897, (Pig) 396823, (Human) 26330, (Pig) 100517097, (Bovine) 532231, (Sheep) 101103587, (Horse) 100051032

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Function(s)

glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity protein binding microtubule binding peptidyl-cysteine S-nitrosylase activity identical protein binding NADP binding NAD binding disordered domain specific binding dehydrogenase

Process(es)

microtubule cytoskeleton organization gluconeogenesis regulation of macroautophagy negative regulation of translation peptidyl-cysteine S-trans-nitrosylation protein stabilization neuron apoptotic process canonical glycolysis cellular response to interferon-gamma glucose metabolic process glycolytic process regulation of translation oxidation-reduction process regulation of neurotransmitter loading into synaptic vesicle spermatid development sperm motility positive regulation of glycolytic process

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GAPDH Monoclonal Antibody (258)

GAPDH Antibody (437000) in ICC/IF

Product Details

437000

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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