OriGene

GAPDH Monoclonal Antibody (OTI2D9), TrueMAB™

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GAPDH Antibody (TA802519) in WB

Figure from citation: Western Blot of GAPDH protein level by using anti-GAPDH antibody in human pancreatic adenocarcinoma cell lines. Dilution: 1:2500 View Citation

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Product Details

TA802519

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

Miscellaneous PubMed (Misc)

View 36 publications 36 publications

Product Specifications

Species Reactivity

Dog, Human, Mouse, Rat

Published species

Not Applicable

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI2D9

Immunogen

Full length human recombinant protein of human GAPDH produced in HEK293T cell.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 1% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, GAPDH is ubiquitously expressed and displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. Studies provide evidence of GAPDH playing an essential part in gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases. Further, GAPDH is involved in other cellular processes ranging from membrane fusion, and neuronal apoptosis in cancer. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease (AD), and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations between GAPDH, actin and tubulin have also be reported. Since GAPDH is expressed at high levels in most tissues, it is useful as protein loading control in Western Blot analysis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Molecular therapy. Nucleic acids

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Fri Mar 01 00:00:00 EST 2019

SIRT1 participates in the response to methylglyoxal-dependent glycative stress in mouse oocytes and ovary.

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Progressive Characterization of Visual Phenotype in Bardet-Biedl Syndrome Mutant Mice.

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Muscle activity prevents the uncoupling of mitochondria from Ca²⁺ Release Units induced by ageing and disuse.

Archives of biochemistry and biophysics

Pietrangelo L,Michelucci A,Ambrogini P,Sartini S,Guarnier FA,Fusella A,Zamparo I,Mammucari C,Protasi F,Boncompagni S

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Aortic constriction induces hypertension and cardiac hypertrophy via (pro)renin receptor activation and the PLC‑β3 signaling pathway.

Molecular medicine reports

Wu J,Zhang C,Liu C,Zhang A,Li A,Zhang J,Zhang Y

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Znhit1 controls intestinal stem cell maintenance by regulating H2A.Z incorporation.

Nature communications

Zhao B,Chen Y,Jiang N,Yang L,Sun S,Zhang Y,Wen Z,Ray L,Liu H,Hou G,Lin X

Wed Mar 06 00:00:00 EST 2019

Efflux excretion of bisdemethoxycurcumin-O-glucuronide in UGT1A1-overexpressing HeLa cells: Identification of breast cancer resistance protein (BCRP) and multidrug resistance-associated proteins 1 (MRP1) as the glucuronide transporters.

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Thu Nov 01 00:00:00 EDT 2018

Trehalose Activates CRE-Dependent Transcriptional Signaling in HT22 Mouse Hippocampal Neuronal Cells: A Central Role for PKA Without cAMP Elevation.

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Maronde E

Tue Sep 29 00:00:00 EDT 2020

The roles of breast cancer resistance protein (BCRP/ABCG2) and multidrug resistance-associated proteins (MRPs/ABCCs) in the excretion of cycloicaritin-3-O-glucoronide in UGT1A1-overexpressing HeLa cells.

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Li S,Xu J,Yao Z,Hu L,Qin Z,Gao H,Krausz KW,Gonzalez FJ,Yao X

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NOD2-RIP2 contributes to the inflammatory responses of mice in vivo to Streptococcus pneumoniae.

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Zheng Y,Shang F,An L,Zhao H,Liu X

Tue Apr 03 00:00:00 EDT 2018

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Wan X,Shi L,Ma X,Tang H,Wu G

Thu Nov 01 00:00:00 EDT 2018

Leucine-rich repeat kinase 2 controls protein kinase A activation state through phosphodiesterase 4.

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SMAD4 feedback regulates the canonical TGF-β signaling pathway to control granulosa cell apoptosis.

Cell death & disease

Du X,Pan Z,Li Q,Liu H,Li Q

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Asperti M,Stuemler T,Poli M,Gryzik M,Lifshitz L,Meyron-Holtz EG,Vlodavsky I,Arosio P

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Shi M,Ma L,Zhou L,Fu P

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TRAF3IP3, a novel autophagy up-regulated gene, is involved in marginal zone B lymphocyte development and survival.

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Cell reports

Zhang D,Wang Y,Shi Z,Liu J,Sun P,Hou X,Zhang J,Zhao S,Zhou BP,Mi J

Tue Mar 03 00:00:00 EST 2015

Novel APP K724M mutation causes Chinese early-onset familial Alzheimer's disease and increases amyloid-β42 to amyloid-β40 ratio.

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Aberrantly Over-Expressed TRPM8 Channels in Pancreatic Adenocarcinoma: Correlation with Tumor Size/Stage and Requirement for Cancer Cells Invasion.

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Biochemical and functional interaction between ZNF224 and ZNF255, two members of the Kruppel-like zinc-finger protein family and WT1 protein isoforms.

Human molecular genetics

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Wed Sep 15 00:00:00 EDT 2010

Reversible conversion from Ca(2)+-ATPase activity to Mg(2)+- and Mn(2)+-ATPase activities of coupling factor purified from acetone powder of Rhodospirillum rubrum chromatophores.

Journal of biochemistry

Soe G,Nishi N,Kakuno T,Yamashita J

Sun Oct 01 00:00:00 EDT 1978

[The periarteritis nodosa--diagnosted by angiography. A case with multiple aneurysms (author's transl)].

Der Radiologe

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Bioinformatics

Protein Aliases: 38 kDa BFA-dependent ADP-ribosylation substrate; aging-associated gene 9 protein; BARS-38; EC 1.2.1.12; epididymis secretory protein Li 278; epididymis secretory sperm binding protein Li 162eP; GAPDH; GAPDH-2; Glyceraldehyde-3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase type 2; Glyceraldehyde-3-phosphate dehydrogenase, testis-specific; HGNC:4141; MGC88685; Peptidyl-cysteine S-nitrosylase GAPDH; Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2; Spermatogenic glyceraldehyde-3-phosphate dehydrogenase

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Gene Aliases: BARS-38; CDABP0047; G3PD; GAPD; Gapd-s; GAPD2; GAPDH; GAPDH-2; GAPDH2; GAPDHS; GAPDS; HEL-S-162eP; HEL-S-278; HSD-35; HSD35; OK/SW-cl.12

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UniProt ID: (Human) P04406, (Human) O14556, (Mouse) P16858, (Rat) P04797, (Rat) Q9ESV6, (Mouse) Q64467

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Entrez Gene ID: (Human) 2597, (Dog) 403755, (Human) 26330, (Dog) 476483, (Mouse) 14433, (Rat) 24383, (Rat) 66020, (Mouse) 14447

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Function(s)

glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity protein binding microtubule binding peptidyl-cysteine S-nitrosylase activity identical protein binding NADP binding NAD binding molecular_function oxidoreductase activity oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor dehydrogenase

Process(es)

microtubule cytoskeleton organization gluconeogenesis regulation of macroautophagy negative regulation of translation peptidyl-cysteine S-trans-nitrosylation protein stabilization neuron apoptotic process canonical glycolysis cellular response to interferon-gamma glucose metabolic process glycolytic process regulation of translation oxidation-reduction process spermatid development sperm motility positive regulation of glycolytic process carbohydrate metabolic process apoptotic process multicellular organism development response to ammonium ion

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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GAPDH Monoclonal Antibody (OTI2D9), TrueMAB™

GAPDH Antibody (TA802519) in WB

Product Details

TA802519

Western Blot (WB)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Target Information

Bioinformatics

Performance Guarantee

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