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GDF3 Antibody (MA5-35968) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of GDF3 in HepG2 cells. Cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with GDF3 Recombinant Monoclonal Antibody (Product # MA5-35968) (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Cytoplasm.
Product Details
MA5-35968
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
010
Immunogen
A synthetic peptide corresponding to the center region of the Human GDF-3
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2866580
Target Information
GDF3 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. The function of this protein is unknown, but expression studies suggest it may be involved in regulation of the adult lymphatic and erythroid systems and embryonic development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GDF-3; Growth/differentiation factor 3
Gene Aliases: GDF3; KFS3; MCOP7; MCOPCB6; UNQ222/PRO248
UniProt ID: (Human) Q9NR23
Entrez Gene ID: (Human) 9573
skeletal system development
eye development
in utero embryonic development
response to dietary excess
endoderm development
mesoderm development
regulation of cell fate commitment
positive regulation of pathway-restricted SMAD protein phosphorylation
negative regulation of BMP signaling pathway
notochord development
somite rostral/caudal axis specification
growth
regulation of apoptotic process
regulation of MAPK cascade
negative regulation of epidermal cell differentiation
negative regulation of myoblast differentiation
cell development
formation of anatomical boundary
SMAD protein signal transduction
primitive streak formation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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