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GDNF Antibody (ANT-014-200UL) in ICC/IF
Expression of GDNF in rat C6 glioma cells - Immunocytochemical staining of GDNF in rat C6 glioma cells. A.Paraformaldehyde-fixed and permeabilized rat C6 glioma cells were stained with Anti-GDNF Antibody (#ANT-014), (1:200) followed by goat Anti-rabbit-AlexaFluor-555 secondary Antibody (red). Nuclei were visualized with the cell-permeable dye Hoechst 33342 (blue). B.Live view of the same field as in (A).
Product Details
ANT-014-200UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
(C)DDNLVYHILRKHSAKR, corresponding to amino acid residues 192-207 of human GDNF (precursor), C-terminus of the mature GDNF
Conjugate
Form
Lyophilized
Concentration
0.8 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Target Information
GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was then shown to rescue these neurons in animal models of Parkinson's disease. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. GDNF is a neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Astrocyte-derived trophic factor; ATF; glial cell line derived neurotrophic factor; Glial cell line derived neutrophic factor; Glial cell line-derived neurotrophic factor; H-GDNF; hGDNF; mGDNF; neurotrophic factor
Gene Aliases: AI385739; ATF1; ATF2; GDNF; gndf; HFB1-GDNF; HSCR3
UniProt ID: (Human) P39905, (Mouse) P48540, (Rat) Q07731
Entrez Gene ID: (Human) 2668, (Mouse) 14573, (Rat) 25453
MAPK cascade
metanephros development
branching involved in ureteric bud morphogenesis
neural crest cell migration
organ induction
postsynaptic membrane organization
mesenchymal to epithelial transition involved in metanephros morphogenesis
signal transduction
nervous system development
positive regulation of cell proliferation
adult locomotory behavior
regulation of gene expression
postganglionic parasympathetic fiber development
peristalsis
neuron projection development
positive regulation of monooxygenase activity
positive regulation of dopamine secretion
negative regulation of apoptotic process
negative regulation of neuron apoptotic process
positive regulation of GTPase activity
positive regulation of cell differentiation
positive regulation of transcription from RNA polymerase II promoter
mRNA stabilization
enteric nervous system development
sympathetic nervous system development
regulation of dopamine uptake involved in synaptic transmission
ureteric bud formation
regulation of morphogenesis of a branching structure
positive regulation of ureteric bud formation
positive regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis
positive regulation of branching involved in ureteric bud morphogenesis
regulation of stem cell differentiation
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
ureteric bud development
transmembrane receptor protein tyrosine kinase signaling pathway
transforming growth factor beta receptor signaling pathway
peripheral nervous system development
cell proliferation
response to wounding
neuron differentiation
cellular response to dexamethasone stimulus
regulation of ureteric bud formation
male gonad development
midbrain development
retina development in camera-type eye
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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