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GIT1 Antibody (MA5-45566) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with GIT1 monoclonal antibody (Product # MA5-45566) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000; 1:5,000 for 60 min RT, 5 min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (... View More
Product Details
MA5-45566
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
N39B/8
Immunogen
Fusion protein amino acids 375-770 (C-terminus) of rat GIT1
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
9.1mM sodium bicarbonate/PBS, pH 7.4, with 581mM DMSO, 123mM ethanolamine
Contains
no preservative
Storage conditions
4° C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2932020
Product Specific Information
1 µg/mL of MA5-45566 was sufficient for detection of GIT1 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 90kDa. Does not cross-react with GIT2.
This antibody was formerly sold as clone S39B-8.
Target Information
GIT1 is a GTPase-activating protein for the ADP ribosylation factor family. It may serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. It increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ARF GAP GIT1; ARF GTPase-activating protein GIT1; CAT-1; CAT1; Cool-associated and tyrosine-phosphorylated protein 1; G protein-coupled receptor kinase interacting ArfGAP 1; G protein-coupled receptor kinase interactor 1; G protein-coupled receptor kinase-associated ADP ribosylation factor GTPase-activating protein; G protein-coupled receptor kinase-associated ADP ribosylation factor GTPase-activating protein (GIT1); G protein-coupled receptor kinase-interactor 1; GRK-interacting protein 1; GRK-interactor 1; p95-APP1
Gene Aliases: Cat-1; GIT1; p95Cat
UniProt ID: (Human) Q9Y2X7, (Mouse) Q68FF6, (Rat) Q9Z272
Entrez Gene ID: (Human) 28964, (Mouse) 216963, (Rat) 83709
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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