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GLB1 Antibody (PA5-65272) in ICC/IF
Immunofluorescence analysis of GLB1 was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with GLB1 Polyclonal Antibody (Product # PA5-65272) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32... View More
Product Details
PA5-65272
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human beta Galactosidase. Recombinant protein control fragment (Product #RP-104284).
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2662594
Product Specific Information
Immunogen sequence: GRYWPARGPQL TLFVPQHILM TSAPNTITVL ELEWAPCSSD DPELCAVTFV DRPVIGSSVT YDHPSKPVEK RLMPPPPQKN KDSWLDHV
Highest antigen sequence identity to the following orthologs - mouse 47%, rat 46%.
Target Information
Beta Galactosidase encodes a member of the glycosyl hydrolase 35 family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature lysosomal enzyme. This enzyme catalyzes the hydrolysis of a terminal beta-linked galactose residue from ganglioside substrates and other glycoconjugates. Mutations in this gene have been associated with GM1-gangliosidosis and Morquio B syndrome. Diseases associated with GLB1 include Gm1-Gangliosidosis, Type I and Gm1-Gangliosidosis, Type Ii.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Acid beta-galactosidase; Beta-Gal; Beta-galactosidase; betagal; bgal; Elastin receptor 1; elastin receptor 1, 67kDa; Lactase; ß-gal; ß-galactosidase; ßgal
Gene Aliases: EBP; ELNR1; GLB1; MPS4B
UniProt ID: (Human) P16278
Entrez Gene ID: (Human) 2720
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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